Dynamic methylation and expression of Oct4 in early neural stem cells.

Journal of Anatomy
Shih-Han LeePaul J Scotting

Abstract

Neural stem cells are a multipotent population of tissue-specific stem cells with a broad but limited differentiation potential. However, recent studies have shown that over-expression of the pluripotency gene, Oct4, alone is sufficient to initiate a process by which these can form 'induced pluripotent stem cells' (iPS cells) with the same broad potential as embryonic stem cells. This led us to examine the expression of Oct4 in endogenous neural stem cells, as data regarding its expression in neural stem cells in vivo are contradictory and incomplete. In this study we have therefore analysed the expression of Oct4 and other genes associated with pluripotency throughout development of the mouse CNS and in neural stem cells grown in vitro. We find that Oct4 is still expressed in the CNS by E8.5, but that this expression declines rapidly until it is undetectable by E15.5. This decline is coincident with the gradual methylation of the Oct4 promoter and proximal enhancer. Immunostaining suggests that the Oct4 protein is predominantly cytoplasmic in location. We also found that neural stem cells from all ages expressed the pluripotency associated genes, Sox2, c-Myc, Klf4 and Nanog. These data provide an explanation for the varying be...Continue Reading

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Citations

Nov 28, 2012·American Journal of Veterinary Research·Ji-Hey LimChristopher L Mariani
Aug 29, 2012·The Journal of Pathology·Chris Tan, Paul J Scotting
Sep 19, 2015·Molecular Neurobiology·Sebastien GabelLuc Grandbarbe
Apr 23, 2015·In Vitro Cellular & Developmental Biology. Animal·Sumiyo MimuraMiho K Furue
Apr 18, 2020·Molecular Autism·Aaron Gordon, Daniel H Geschwind
Jul 5, 2011·Journal of Neuro-oncology·Carolina Oliveira RodiniOswaldo Keith Okamoto
Jan 21, 2021·Cell & Bioscience·Liyang XuYing Cao
May 5, 2020·Current Cancer Drug Targets·Sekaran BalajiAyyasamy Vanniarajan

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