Dynamic microfluidic nanocalorimetry system for measuring Caenorhabditis elegans metabolic heat

Lab on a Chip
Roger KrengerMartin A M Gijs

Abstract

Basal heat production is a key phenotype for assessing the metabolic activity of small living organisms. Here, we present a new nanocalorimetric system, based on thin film thermopile sensors combined with microfluidic chips for measuring metabolic heat signals generated by Caenorhabditis elegans larval populations (60 to 220 organisms). In addition to versatile on-chip fluidic manipulation, our microfluidic approach allows confining worm populations close to the sensor surface, thus increasing the sensitivity of the assays. A customized flow protocol for dynamically displacing the worm population on-chip and off-chip was applied. The resulting sequential recordings of heat source and reference signals enabled precise measurements of slow varying heat-generating metabolic processes. We found an increase of the volume-specific basal heat production from the L2 to the L3 larval stage, and a significant decrease from the L3 to the L4 stage. Additionally, we investigated the metabolic heat production of the larval populations during maximal respiratory capacity, i.e. after inducing uncoupled respiration by on-chip treatment with the mitochondrial uncoupling agent carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP). Depending...Continue Reading

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Citations

May 17, 2019·Analytical and Bioanalytical Chemistry·Johannes LerchnerAnibal E Vercesi
Feb 11, 2020·Biomicrofluidics·Shuyu WangYuliang Zhao
Jul 22, 2019·Sensors·Manuel Domínguez-PumarJoan Pons-Nin
May 10, 2020·Microsystems & Nanoengineering·Roger KrengerMartin A M Gijs
Sep 13, 2020·Science Advances·Masazumi FujiwaraEriko Kage-Nakadai
Jun 14, 2020·Nature Communications·Sahngki HongRenkun Chen

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Datasets Mentioned

BETA
GM3025
GM3050

Methods Mentioned

BETA
isothermal
by
chip
biosensors
chips

Software Mentioned

MATLAB

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