Dynamic monitoring of apoptosis in chemotherapies with multiple fluorescence reporters.

Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging
Yunlong LiQun Chen

Abstract

The aim of the study is to dynamically and non-invasively monitor the temporal relationship among caspase-3, BID, and cytochrome c in chemotherapy. ASTC-a-1 cells expressing the corresponding fluorescence reporters were treated with Taxol or cisplatin and imaged using FRET and fluorescence overlapping technique. Western blot was performed to validate the fluorescence analysis. In fluorescence imaging analysis, Taxol-induced apoptosis showed caspase-3 activation (13 h 50 min) was prior to BID cleavage (15 h 10 min) and subsequent significant cytochrome c release (17-18 h 20 min), whereas the cisplatin-induced apoptosis showed BID cleavage (5 h 40 min) and significant cytochrome c release (7-8 h 20 min) were prior to caspase-3 activation (14 h 20 min). Western blot further validated the results above. The new approach successfully reveals the difference in temporal signaling apoptosis events between Taxol and cisplatin. It may help us come to a better understanding of the detailed mechanisms in chemotherapeutic-agents-induced apoptosis.

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Citations

Jun 23, 2009·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Feifan ZhouWei R Chen
Dec 3, 2011·Journal of Cellular Biochemistry·Chaoyang Wang, Tongsheng Chen
Feb 17, 2015·Cancer Cell International·Michael JelínekJan Kovář
Nov 13, 2009·International Journal of Cancer. Journal International Du Cancer·Yunlong LiWei R Chen

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis