Dynamic monitoring of NET activity in mature murine sympathetic terminals using a fluorescent substrate.

British Journal of Pharmacology
Lauren K ParkerK L Brain

Abstract

To validate a fluorescence approach for monitoring norepinephrine transporter (NET) transport rate in mature sympathetic terminals, and to determine how prejunctional muscarinic receptors affect NET rate. Confocal imaging of a fluorescent NET substrate [neurotransmitter transporter uptake assay (NTUA)] as it accumulates in the mature sympathetic nerve terminals of the mouse isolated vas deferens. Fluorescence recovery after photobleaching (FRAP), enhanced green fluorescence protein (EGFP)-transgenic mice and contraction studies were also used. NTUA fluorescence accumulated linearly in nerve terminals, an effect that was prevented with NET inhibition with desipramine (1 microM). Such accumulation was reversed by amphetamine (10 microM), which is known to reverse the direction of transport of NET substrates. NTUA labelling was not present in cholinergic terminals (identified using EGFP fluorescence expressed in transgenic mice under a choline acetyltransferase promoter). FRAP experiments, altered nerve terminal distribution with reserpine pretreatment and co-imaging in terminals filled with a cytoplasmic marker (Alexa 594 dextran) indicated that the NTUA labelling was largely confined to vesicles within varicosities; vesicular ex...Continue Reading

References

Jan 1, 1975·Naunyn-Schmiedeberg's Archives of Pharmacology·S B Ross, T Gosztonyi
Nov 17, 1998·Microscopy Research and Technique·K Kihara
Nov 17, 1998·Microscopy Research and Technique·K KiharaH Oshima
Feb 24, 2000·The New England Journal of Medicine·J R ShannonD Robertson
Feb 7, 2001·The Journal of Comparative Neurology·S SchroeterR D Blakely
Aug 7, 2003·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Ersin YarisRasin Ozyavuz
Jul 12, 2005·Cardiovascular Research·Johannes BacksMarkus Haass
Sep 20, 2005·British Journal of Pharmacology·Alina M CuprianThomas C Cunnane
Nov 25, 2006·Kidney & Blood Pressure Research·Piotr KsiazekMonika Buraczynska
Dec 26, 2006·Molecular and Cellular Neurosciences·Uhna Sung, Randy D Blakely
May 2, 2008·British Journal of Pharmacology·S P H AlexanderJ A Peters

❮ Previous
Next ❯

Citations

Jan 2, 2013·Proceedings of the National Academy of Sciences of the United States of America·Pamela C RodriguezDavid Sulzer
Feb 1, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Maria BorisovskaGary L Westbrook
Jul 28, 2011·British Journal of Pharmacology·Andrew J Lawrence, Andreas Heinz
Apr 15, 2010·British Journal of Pharmacology·Anthony P Davenport, Craig Daly
Dec 3, 2014·The Veterinary Journal·Hannah KenwardJonathan Elliott
Sep 6, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Julia Shanks, Neil Herring
Jun 19, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Junpei KobayashiMasashi Aoki
Mar 15, 2021·Autonomic Neuroscience : Basic & Clinical·Lily L CaoKeith L Brain

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.