Dynamic motions of free and bound O29 scaffolding protein identified by hydrogen deuterium exchange mass spectrometry

Protein Science : a Publication of the Protein Society
Chi-Yu Fu, Peter E Prevelige

Abstract

In the double-stranded DNA containing bacteriophages, hundreds of copies of capsid protein subunits polymerize to form icosahedral shells, called procapsids, into which the viral genome is subsequently packaged to form infectious virions. High assembly fidelity requires the assistance of scaffolding protein molecules, which interact with the capsid proteins to insure proper geometrical incorporation of subunits into the growing icosahedral lattices. The interactions between the scaffolding and capsid proteins are transient and are subsequently disrupted during DNA packaging. Removal of scaffolding protein is achieved either by proteolysis or alternatively by some form of conformational switch that allows it to dissociate from the capsid. To identify the switch controlling scaffolding protein association and release, hydrogen deuterium exchange was applied to Bacillus subtilis phage Ø29 scaffolding protein gp7 in both free and procapsid-bound forms. The H/D exchange experiments revealed highly dynamic and cooperative opening motions of scaffolding molecules in the N-terminal helix-loop-helix (H-L-H) region. The motions can be promoted by destabilizing the hydrophobic contact between two helices. At low temperature where high ene...Continue Reading

Citations

Aug 1, 2006·Journal of the American Society for Mass Spectrometry·David D WeisLynn F Ten Eyck
May 5, 2012·Protein Expression and Purification·Ansgar Brock
Jan 3, 2007·Journal of Molecular Biology·Chi-yu FuPeter E Prevelige
Dec 22, 2016·Journal of Structural Biology·Ji Young ParkKa Young Chung
Jan 3, 2007·Journal of Mass Spectrometry : JMS
May 24, 2008·Mass Spectrometry Reviews·Victoria L MortonAlison E Ashcroft
Jul 18, 2008·Rapid Communications in Mass Spectrometry : RCM·Anton PoliakovPeter E Prevelige
Nov 5, 2020·Protein and Peptide Letters·Min Woo YunKa Young Chung

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