Dynamic organisation of prolactin gene expression in living pituitary tissue.

Journal of Cell Science
Claire V HarperMichael R H White

Abstract

Gene expression in living cells is highly dynamic, but temporal patterns of gene expression in intact tissues are largely unknown. The mammalian pituitary gland comprises several intermingled cell types, organised as interdigitated networks that interact functionally to generate co-ordinated hormone secretion. Live-cell imaging was used to quantify patterns of reporter gene expression in dispersed lactotrophic cells or intact pituitary tissue from bacterial artificial chromosome (BAC) transgenic rats in which a large prolactin genomic fragment directed expression of luciferase or destabilised enhanced green fluorescent protein (d2EGFP). Prolactin promoter activity in transgenic pituitaries varied with time across different regions of the gland. Although amplitude of transcriptional responses differed, all regions of the gland displayed similar overall patterns of reporter gene expression over a 50-hour period, implying overall co-ordination of cellular behaviour. By contrast, enzymatically dispersed pituitary cell cultures showed unsynchronised fluctuations of promoter activity amongst different cells, suggesting that transcriptional patterns were constrained by tissue architecture. Short-term, high resolution, single cell anal...Continue Reading

References

Mar 1, 1987·Endocrinology·F R Boockfor, L S Frawley
Jan 1, 1970·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P K Nakane
Jan 1, 1997·Experimental and Clinical Endocrinology & Diabetes : Official Journal, German Society of Endocrinology [and] German Diabetes Association·U RennerG K Stalla
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·T FauquierP Mollard
Dec 12, 2001·Endocrine Reviews·N Ben-Jonathan, R Hnasko
Mar 8, 2002·The Journal of Physiology·H C Christian, J F Morris
Jan 30, 2003·Molecular Endocrinology·A J NorrisJ R E Davis
Nov 2, 2004·Journal of Microscopy·G Rabut, J Ellenberg
Nov 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Xavier BonnefontPatrice Mollard
Oct 4, 2006·Journal of the Royal Society, Interface·Hailin ShenDouglas B Kell
Aug 22, 2007·Seminars in Reproductive Medicine·Stephen J Winters, Joseph P Moore
Dec 18, 2007·Journal of Neuroendocrinology·C Denef
Oct 30, 2008·Cell·Arjun Raj, Alexander van Oudenaarden
Mar 24, 2009·Journal of Neuroendocrinology·L A Nolan, A Levy

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Citations

Apr 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·Tetsuya MuramotoJonathan R Chubb
Oct 11, 2011·Journal of Cell Science·Karen FeatherstoneJulian R E Davis
May 3, 2011·PLoS Biology·Claire V HarperMichael R H White
Feb 2, 2016·ELife·Karen FeatherstoneJulian R E Davis
Mar 23, 2012·Trends in Endocrinology and Metabolism : TEM·Patrice MollardJacques Drouin
Aug 2, 2011·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jean-Pascal Capp
Jan 11, 2012·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jean-Pascal Capp
Feb 21, 2016·ELife·Adam M CorriganJonathan R Chubb
Mar 31, 2015·Biostatistics·Kirsty L HeyBärbel Finkenstädt
Dec 6, 2011·Current Opinion in Biotechnology·Denise BakstadMichael R H White
Sep 17, 2011·Developmental Cell·Margaret E Buckingham, Sigolène M Meilhac
Sep 18, 2012·Frontiers in Neuroendocrinology·P R Le TissierP Mollard
Dec 10, 2016·Nature Reviews. Endocrinology·Paul Le TissierPatrice Mollard
Jan 5, 2017·International Journal of Cancer. Journal International Du Cancer·Jean-Pascal Capp
Jun 18, 2017·Nucleus·M HornáčekE Smirnov
Jun 11, 2010·Nature·David G SpillerMichael R H White
May 5, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Akira MatsunoR Yoshiyuki Osamura
Dec 30, 2017·Nucleus·Evgeny SmirnovIvan Raška
Dec 23, 2015·Molecular Endocrinology·Anne V McNamaraMichael R H White
Jun 14, 2019·PLoS Computational Biology·Hiroshi MomijiDavid A Rand
Feb 1, 2017·The EMBO Journal·Aneta Koseska, Philippe Ih Bastiaens
Jun 29, 2012·Biomedical Optics Express·C D SaunterJ M Girkin
Jan 4, 2012·Annals of the New York Academy of Sciences·Julian R E Davis
Mar 22, 2018·Diabetes·Richard K P Benninger, David J Hodson

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