Dynamic oxidative stress and DNA damage induced by oestrogen deficiency and protective effects of puerarin and 17β-oestradiol in ovariectomized rats

Basic & Clinical Pharmacology & Toxicology
Xu-Lei TangWei Zhang

Abstract

Oxidative stress plays an essential role in the pathogenesis of cardiovascular diseases and osteoporosis resulting from oestrogen deficiency in the postmenopausal period. In this report, we observed a dynamic change of oxidative stress and DNA damage after ovariectomy in female rats. We then compared phytoestrogen puerarin and 17β-oestradiol (E₂) in their effects on oestrogen deficiency-induced oxidative stress and DNA damage. Serum total antioxidant capacity (TAC), malondialdehyde (MDA) and lymphocytes DNA damage (comet%) were measured. There was a gradual increase in oxidative stress in the ovariectomized (OVX) rats over time after ovariectomy, as compared to rats receiving sham operation. OVX rats that were on puerarin and E₂ showed increased TAC and decreased MDA in the serum, as well as decreased lymphocytes comet%. Puerarin appeared to have a more powerful protective effect on DNA oxidative damage than E₂. The study indicates that postmenopausal women may benefit from phytoestrogen puerarin.

References

Jun 1, 1979·Analytical Biochemistry·H OhkawaK Yagi
Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
Mar 31, 1999·Free Radical Research·C M GedikA R Collins
Jan 8, 2000·Stroke; a Journal of Cerebral Circulation·J S LeinonenH Alho
Jun 23, 2000·Mutation Research·S M EverettA T Axon
Sep 8, 2000·Stroke; a Journal of Cerebral Circulation·M A Topcuoglu, S Demirkaya
Nov 23, 2000·Nature·T Finkel, N J Holbrook
Nov 28, 2001·Heart & Lung : the Journal of Critical Care·D W Schwertz, S Penckofer
Jun 7, 2002·Endocrine Reviews·B Lawrence RiggsL Joseph Melton
Apr 29, 2004·Early Human Development·Jin ZhaoRong-Liang Zheng
Jun 10, 2004·Journal of Agricultural and Food Chemistry·Jeevan K PrasainStephen Barnes
Dec 3, 2005·European Journal of Pharmacology·Fu-Liang XiongHui-Bi Xu
Apr 29, 2006·Menopause : the Journal of the North American Menopause Society·Kwok-Keung LamMao-Hsiung Yen
Aug 5, 2008·Cell Biology International·Hai-Ying ZhangHai-Tao Hu
Oct 10, 2008·Planta medica·Tawakalitu OseniV Craig Jordan
Jun 26, 2009·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Antonella ZucchettoRenato Talamini
Dec 5, 2009·Aging Clinical and Experimental Research·Necat Yilmaz, Esin Eren
Feb 2, 2010·Fitoterapia·Suchinda MalaivijitnondNathachit Limjunyawong
Jul 8, 2010·Archives of Pharmacal Research·Sun Ju KimYoung Chul Kim
Jan 20, 2011·Clinical Science·Douglas R SealsAnthony J Donato

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Citations

Apr 11, 2014·International Journal of Biological Sciences·Yang LiuXiaoyu Xu
Dec 18, 2013·Phytotherapy Research : PTR·Yan-Xi ZhouCheng Peng

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