Dynamic patterns of subcellular protein localization during spore coat morphogenesis in Bacillus subtilis

Journal of Bacteriology
Christiaan van OoijR Losick

Abstract

Endospores of Bacillus subtilis are encased in a thick, proteinaceous shell known as the coat, which is composed of a large number of different proteins. Here we report the identification of three previously uncharacterized coat-associated proteins, YabP, YheD, and YutH, and their patterns of subcellular localization during the process of sporulation, obtained by using fusions of the proteins to the green fluorescent protein (GFP). YabP-GFP was found to form both a shell and a ring around the center of the forespore across the short axis of the sporangium. YheD-GFP, in contrast, formed two rings around the forespore that were offset from its midpoint, before it eventually redistributed to form a shell around the developing spore. Finally, YutH-GFP initially localized to a focus at one end of the forespore, which then underwent transformation into a ring that was located adjacent to the forespore. Next, the ring became a cap at the mother cell pole of the forespore that eventually spread around the entire developing spore. Thus, each protein exhibited its own distinct pattern of subcellular localization during the course of coat morphogenesis. We concluded that spore coat assembly is a dynamic process involving diverse patterns ...Continue Reading

References

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Jun 28, 2000·Proceedings of the National Academy of Sciences of the United States of America·P FawcettP Youngman
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Feb 4, 2003·Journal of Bacteriology·Christiaan van Ooij, Richard Losick
Feb 4, 2003·Journal of Bacteriology·Erh-Min LaiAdam Driks
Mar 29, 2003·Journal of Molecular Biology·Patrick EichenbergerRichard Losick

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Citations

Aug 12, 2010·Journal of the American Chemical Society·Yunhuang YangMichael A Kennedy
Aug 17, 2010·Journal of Bacteriology·Matthias SchmalischRichard Losick
Nov 26, 2009·Journal of Bacteriology·Daisuke ImamuraKazuhito Watabe
Nov 6, 2007·Journal of Bacteriology·Gonçalo RealJonathan Dworkin
Nov 18, 2004·Journal of Bacteriology·Jessica M SilvaggiRichard Losick
Dec 3, 2005·Journal of Bacteriology·D C McPhersonA Driks
Nov 24, 2007·Annual Review of Microbiology·Adriano O Henriques, Charles P Moran
Dec 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Lawrence A KlobutcherPeter Setlow
Nov 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Himatkumar V PatelSaul Roseman
Sep 25, 2009·Molecular Microbiology·Katherine H WangPatrick Eichenberger
Jan 5, 2006·Molecular Microbiology·Hosan KimAdam Driks
Dec 17, 2011·Molecular Microbiology·Peter T McKenney, Patrick Eichenberger
May 7, 2010·Molecular Microbiology·Kerwyn Casey Huang, Kumaran S Ramamurthi
Feb 25, 2006·Journal of Molecular Biology·Stephanie T WangPatrick Eichenberger
Mar 9, 2005·Current Biology : CB·Kumaran S Ramamurthi, Richard M Losick
Oct 30, 2016·Frontiers in Microbiology·Wishwas R AbhyankarLeo J de Koning
Dec 4, 2012·Nature Reviews. Microbiology·Peter T McKenneyPatrick Eichenberger
Dec 15, 2020·PLoS Genetics·Johana LuhurChristopher D A Rodrigues
Feb 13, 2021·Microorganisms·Daniela KrajčíkováImrich Barák
May 27, 2016·Microbiology Spectrum·Adam Driks, Patrick Eichenberger

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