Dynamic pH-induced conformational changes of the PsbO protein in the fluctuating acidity of the thylakoid lumen

Physiologia Plantarum
Anke B CariusTatyana Shutova

Abstract

The PsbO protein is an essential extrinsic subunit of photosystem II, the pigment-protein complex responsible for light-driven water splitting. Water oxidation in photosystem II supplies electrons to the photosynthetic electron transfer chain and is accompanied by proton release and oxygen evolution. While the electron transfer steps in this process are well defined and characterized, the driving forces acting on the liberated protons, their dynamics and their destiny are all largely unknown. It was suggested that PsbO undergoes proton-induced conformational changes and forms hydrogen bond networks that ensure prompt proton removal from the catalytic site of water oxidation, i.e. the Mn4 CaO5 cluster. This work reports the purification and characterization of heterologously expressed PsbO from green algae Chlamydomonas reinhardtii and two isoforms from the higher plant Solanum tuberosum (PsbO1 and PsbO2). A comparison to the spinach PsbO reveals striking similarities in intrinsic protein fluorescence and CD spectra, reflecting the near-identical secondary structure of the proteins from algae and higher plants. Titration experiments using the hydrophobic fluorescence probe ANS revealed that eukaryotic PsbO proteins exhibit acid-...Continue Reading

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Citations

Apr 24, 2019·Physiologia Plantarum·Anja Krieger-LiszkayJohannes Messinger
Nov 21, 2020·Physiologia Plantarum·Anna PodmaniczkiSzilvia Z Tóth
Sep 14, 2019·Biochimica Et Biophysica Acta. Bioenergetics·Haijun Liu, Robert E Blankenship
Apr 29, 2021·European Biophysics Journal : EBJ·Ria K BaloghBéla Gyurcsik

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