Dynamic phase differences based on quantitative phase imaging for the objective evaluation of cell behavior

Journal of Biomedical Optics
Aneta KrizovaRadim Chmelik

Abstract

Quantitative phase imaging (QPI) brought innovation to noninvasive observation of live cell dynamics seen as cell behavior. Unlike the Zernike phase contrast or differential interference contrast, QPI provides quantitative information about cell dry mass distribution. We used such data for objective evaluation of live cell behavioral dynamics by the advanced method of dynamic phase differences (DPDs). The DPDs method is considered a rational instrument offered by QPI. By subtracting the antecedent from the subsequent image in a time-lapse series, only the changes in mass distribution in the cell are detected. The result is either visualized as a two dimensional color-coded projection of these two states of the cell or as a time dependence of changes quantified in picograms. Then in a series of time-lapse recordings, the chain of cell mass distribution changes that would otherwise escape attention is revealed. Consequently, new salient features of live cell behavior should emerge. Construction of the DPDs method and results exhibiting the approach are presented. Advantage of the DPDs application is demonstrated on cells exposed to an osmotic challenge. For time-lapse acquisition of quantitative phase images, the recently develop...Continue Reading

References

Jul 11, 2006·Journal of Biomedical Optics·Björn KemperGert von Bally
Feb 12, 2008·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·J StrandA K Jain
Jun 20, 2008·American Journal of Physiology. Cell Physiology·Gabriel PopescuKamran Badizadegan
Aug 7, 2006·Optics Express·Florian CharrièreBenjamin Rappaz
Oct 14, 2010·Optics Express·Pavel Kolman, Radim Chmelík
Oct 2, 2012·Biomedical Optics Express·Stefan WitteMarie Louise Groot

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Citations

Aug 25, 2017·Journal of Biomedical Optics·Lenka StrbkovaRadim Chmelik
Mar 16, 2017·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Mikhail E KandelGabriel Popescu

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