Dynamic polar sequestration of excess MurG may regulate enzymatic function.

Journal of Bacteriology
Allison M Michaelis, Zemer Gitai

Abstract

Advances in bacterial cell biology have demonstrated the importance of protein localization for protein function. In general, proteins are thought to localize to the sites where they are active. Here we demonstrate that in Escherichia coli, MurG, the enzyme that mediates the last step in peptidoglycan subunit biosynthesis, becomes polarly localized when expressed at high cellular concentrations. MurG only becomes polarly localized at levels that saturate MurG's cellular requirement for growth, and E. coli cells do not insert peptidoglycan at the cell poles, indicating that the polar MurG is not active. Fluorescence recovery after photobleaching (FRAP) and single-cell biochemistry experiments demonstrate that polar MurG is dynamic. Polar MurG foci are distinct from inclusion body aggregates, and polar MurG can be remobilized when MurG levels drop. These results suggest that polar MurG represents a temporary storage mechanism for excess protein that can later be remobilized into the active pool. We investigated and ruled out several candidate pathways for polar MurG localization, including peptidoglycan biosynthesis, the MreB cytoskeleton, and polar cardiolipin, as well as MurG enzymatic activity and lipid binding, suggesting tha...Continue Reading

References

Mar 1, 1993·Journal of Bacteriology·K Bupp, J van Heijenoort
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·J PoglianoJ Beckwith
May 1, 1997·Journal of Bacteriology·M A de PedroH Schwarz
Apr 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·J V Höltje
May 17, 2000·Proceedings of the National Academy of Sciences of the United States of America·D YuD L Court
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Jun 19, 2003·Journal of Bacteriology·Els van den Brink-van der LaanBen de Kruijff
Jun 26, 2004·Advances in Biochemical Engineering/biotechnology·Beatrix FahnertPeter Neubauer
Jun 26, 2004·Advances in Biochemical Engineering/biotechnology·Aleksei Rozkov, Sven-Olof Enfors
Apr 12, 2006·Genome Research·Mohammad ArifuzzamanHirotada Mori
May 17, 2007·Molecular Microbiology·Tatyana RomantsovJanet M Wood
Aug 19, 2007·Biochimie·Muriel CrouvoisierDominique Mengin-Lecreulx
Oct 6, 2007·Journal of Neuropathology and Experimental Neurology·Charleen T ChuKelly L Jordan-Sciutto
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ariel B LindnerFrançois Taddei
Apr 9, 2008·Biophysical Journal·Ranjan MukhopadhyayNed S Wingreen
May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·John N WernerZemer Gitai
Jul 15, 2009·Journal of Molecular Biology·Assaf RokneyAmos B Oppenheim

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Citations

Sep 19, 2012·Proceedings of the National Academy of Sciences of the United States of America·Brandon K TanZiqiang Guan
Jun 29, 2011·Journal of Molecular Microbiology and Biotechnology·Frédérique PompeoAnne Galinier
Jul 18, 2014·ELife·Rachael M BarryZemer Gitai
Aug 19, 2015·Computational Biology and Chemistry·Stacey M Gifford, Pablo Meyer
May 9, 2012·Biochimica Et Biophysica Acta·Rodrigo Arias-CartinAxel Magalon
Nov 13, 2012·Current Opinion in Microbiology·Hélène Bierne, Shaynoor Dramsi
Jul 19, 2019·Proceedings of the National Academy of Sciences of the United States of America·Xiaofeng ZhouLucy Shapiro
Dec 31, 2020·Proceedings of the National Academy of Sciences of the United States of America·Tamar SzokeOrna Amster-Choder

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