Dynamic quantification of MHC class I-peptide presentation to CD8+ T cells via intracellular cytokine staining

Journal of Immunological Methods
Ken C PangWeisan Chen

Abstract

In order to further our basic understanding of antigen processing and presentation as well as to translate that knowledge into clinically effective vaccines and immunotherapies, having appropriate tools to study MHC class I-peptide presentation is highly desirable. Current methods are based upon HPLC fractionation of extracted peptides, monoclonal Ab, multivalent T cell receptors (TCR), T cell hybridomas, TCR transgenic cells, and T cell lines. However, each of these is associated with problems that make them either difficult to apply generally or too insensitive to adequately quantitate antigen presentation. We have developed a method based upon intracellular cytokine staining (ICS) that dynamically and relatively quantitates MHC class I-peptide presentation to CD8+ T cells in a manner that is both widely applicable and highly sensitive. It is well-suited to assess antigen presentation in its early stages, does not require fixation nor labeling of antigen presenting cells (APC), can be used to examine cross-presentation, and is able to directly employ ex vivo T cells which obviates the need for the development and maintenance of T cell lines and hybridomas. Our method represents a simple yet powerful tool that others intereste...Continue Reading

References

Mar 1, 1994·International Immunology·S Sanderson, N Shastri
Feb 26, 1993·Journal of Immunological Methods·T JungC Rieger
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·M A Alexander-MillerJ A Berzofsky
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·P S AndersenS Buus
Oct 29, 2003·Journal of Immunological Methods·Michael R BettsRichard A Koup
Oct 29, 2003·Journal of Immunological Methods·David H CanadayW Henry Boom
Jan 22, 2004·Current Opinion in Immunology·Nicholas R J Gascoigne, Tomasz Zal
Jul 15, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ian D DavisJonathan S Cebon

❮ Previous
Next ❯

Citations

Feb 6, 2013·PLoS Pathogens·Nathan P CroftAnthony W Purcell
May 16, 2008·Expert Opinion on Therapeutic Targets·Liu Hong, Daiming Fan
Aug 28, 2007·Journal of Immunological Methods·Petra ZelnickovaFrantisek Kovaru
Dec 13, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fabio LucianiWeisan Chen
May 28, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Damien ZankerWeisan Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.