Dynamic regulation of a cell adhesion protein complex including CADM1 by combinatorial analysis of FRAP with exponential curve-fitting

PloS One
Mika Sakurai-YagetaYoshinori Murakami

Abstract

Protein components of cell adhesion machinery show continuous renewal even in the static state of epithelial cells and participate in the formation and maintenance of normal epithelial architecture and tumor suppression. CADM1 is a tumor suppressor belonging to the immunoglobulin superfamily of cell adhesion molecule and forms a cell adhesion complex with an actin-binding protein, 4.1B, and a scaffold protein, MPP3, in the cytoplasm. Here, we investigate dynamic regulation of the CADM1-4.1B-MPP3 complex in mature cell adhesion by fluorescence recovery after photobleaching (FRAP) analysis. Traditional FRAP analysis were performed for relatively short period of around 10 min. Here, thanks to recent advances in the sensitive laser detector systems, we examine FRAP of CADM1 complex for longer period of 60 min and analyze the recovery with exponential curve-fitting to distinguish the fractions with different diffusion constants. This approach reveals that the fluorescence recovery of CADM1 is fitted to a single exponential function with a time constant (τ) of approximately 16 min, whereas 4.1B and MPP3 are fitted to a double exponential function with two τs of approximately 40-60 sec and 16 min. The longer τ is similar to that of CA...Continue Reading

References

Oct 1, 1982·Biophysical Journal·J YguerabideE E Yguerabide
Aug 7, 1999·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·C Harken JensenB Teisner
Mar 14, 2000·Current Opinion in Cell Biology·K B Hoover, P J Bryant
Mar 30, 2001·Nature Genetics·M KuramochiY Murakami
Jun 7, 2002·The Journal of Biological Chemistry·Mari MasudaYoshinori Murakami
Aug 31, 2002·Science·Thomas BiedererThomas C Südhof
Feb 26, 2003·Cell·Mirna Perez-MorenoElaine Fuchs
Jun 17, 2003·The Journal of Biological Chemistry·Toshihiko HanadaAthar H Chishti
Jun 11, 2004·Biophysical Journal·Brian L SpragueJames G McNally
Sep 10, 2005·International Journal of Cancer. Journal International Du Cancer·Daisuke YamadaYoshinori Murakami
Oct 15, 2005·The Journal of Biological Chemistry·Mari MasudaYoshinori Murakami
Dec 6, 2005·Cell·Soichiro YamadaW James Nelson
Feb 24, 2006·Nature Reviews. Molecular Cell Biology·Jean Paul Thiery, Jonathan P Sleeman
Nov 7, 2006·Developmental Cell·Mirna Perez-Moreno, Elaine Fuchs
May 16, 2008·Nature·Matthieu CaveyThomas Lecuit
May 20, 2008·Biophysical Journal·Minchul Kang, Anne K Kenworthy
Nov 4, 2008·Nature Cell Biology·Matteo RauziPierre-François Lenne
Apr 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Simon de BecoSylvie Coscoy
Sep 29, 2009·Development·Adam GiangrecoFiona M Watt
Oct 27, 2009·Biochemical and Biophysical Research Communications·Mika Sakurai-YagetaYoshinori Murakami
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Soonjin HongSergey M Troyanovsky
Apr 29, 2011·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Yuka TakahashiYoshinori Murakami
Dec 14, 2011·Journal of Cell Science·Juan HuangYang Hong

❮ Previous
Next ❯

Citations

Aug 23, 2018·Frontiers in Cell and Developmental Biology·Takeshi ItoYoshinori Murakami
Mar 27, 2019·Cell Death & Disease·Edward J HartsoughAndrew E Aplin
Mar 15, 2018·Biophysical Journal·James L KingsleyErkan Tüzel

❮ Previous
Next ❯

Methods Mentioned

BETA
Fluorescence
transfection
PCR
confocal microscopy

Software Mentioned

Sigma Plot
OriginPro
OriginLab

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Actin-binding Proteins

Actin-binding proteins are a component of the actin cytoskeleton that play essential roles in cellular functions such as regulation of actin polymerization, maintenance of cell polarity, gene expression regulation, cell motility and many more functions. Discover the latest research on actin-binding proteins here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Cell Adhesion Molecules in AS

Cell adhesion molecules expressed on the vascular endothelium and circulating leukocytes in response to inflammatory stimuli are implicated in atherosclerosis. Here is the latest research.