Dynamic regulation of Pep-induced immunity through post-translational control of defence transcript splicing.

Nature Plants
Keini DressanoAlisa Huffaker

Abstract

The survival of all living organisms requires the ability to detect attacks and swiftly counter them with protective immune responses. Despite considerable mechanistic advances, the interconnectivity of signalling modules often remains unclear. A newly characterized protein, IMMUNOREGULATORY RNA-BINDING PROTEIN (IRR), negatively regulates immune responses in both maize and Arabidopsis, with disrupted function resulting in enhanced disease resistance. IRR associates with and promotes canonical splicing of transcripts encoding defence signalling proteins, including the key negative regulator of pattern-recognition receptor signalling complexes, CALCIUM-DEPENDENT PROTEIN KINASE 28 (CPK28). On immune activation by Plant Elicitor Peptides (Peps), IRR is dephosphorylated, disrupting interaction with CPK28 transcripts and resulting in the accumulation of an alternative splice variant encoding a truncated CPK28 protein with impaired kinase activity and diminished function as a negative regulator. We demonstrate a new mechanism linking Pep-induced post-translational modification of IRR with post-transcriptionally mediated attenuation of CPK28 function to dynamically amplify Pep signalling and immune output.

References

Jul 1, 1996·Genes & Development·J L Manley, R Tacke
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·T JabsD Scheel
Dec 14, 1999·The Journal of Biological Chemistry·M Golovkin, A S Reddy
Feb 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·S P Dinesh-Kumar, B J Baker
Mar 5, 2002·Nature·Tsuneaki AsaiJen Sheen
Nov 5, 2003·Journal of Leukocyte Biology·Koichi S Kobayashi, Richard A Flavell
Jun 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yube YamaguchiClarence A Ryan
Jun 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Alisa HuffakerClarence A Ryan
Apr 11, 2007·The Plant Journal : for Cell and Molecular Biology·Biswa R AcharyaRamesh Raina
May 31, 2007·PloS One·Gul Shad AliAnireddy S N Reddy
Jul 27, 2007·The Plant Journal : for Cell and Molecular Biology·Thomas S NühseScott C Peck
Dec 19, 2009·European Journal of Cell Biology·Sandra PostelThorsten Nürnberger
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Dongping LuPing He
Jan 28, 2010·The Journal of Biological Chemistry·Birgit SchulzeDelphine Chinchilla
Jun 28, 2011·Current Opinion in Plant Biology·Guillaume TenaJen Sheen
Jul 27, 2011·PloS One·Fran SupekTomislav Šmuc
Nov 6, 2012·Biochimica Et Biophysica Acta·Anja Liese, Tina Romeis
Feb 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Zixu LiuJian-Min Zhou
Feb 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Nico TintorYusuke Saijo
Mar 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Alisa HuffakerEric A Schmelz
Nov 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Justin W WalleySteven P Briggs
Dec 21, 2013·The EMBO Journal·Annegret RossYusuke Saijo
Mar 25, 2014·Seminars in Cell & Developmental Biology·Minna-Liisa Änkö
Apr 29, 2014·Molecular Cell·Alberto P Macho, Cyril Zipfel
May 24, 2014·Nature Reviews. Immunology·Susan CarpenterKatherine A Fitzgerald
Jun 12, 2014·International Journal of Molecular Sciences·Shengming YangHongyan Zhu
Dec 20, 2014·Cell Host & Microbe·Jacqueline MonaghanCyril Zipfel
Aug 27, 2015·The Plant Journal : for Cell and Molecular Biology·Zhihao LingShuqing Xu
Jul 22, 2016·Immunity·Juan LiuXuetao Cao
Aug 20, 2016·Science·Justin W WalleySteven P Briggs

❮ Previous
Next ❯

Citations

Jul 22, 2020·Nature Plants·Lennart Wirthmueller, Tina Romeis
Oct 16, 2020·The Plant Journal : for Cell and Molecular Biology·Elly PoretskyAlisa Huffaker
May 5, 2021·Proceedings of the National Academy of Sciences of the United States of America·Melissa BredowJacqueline Monaghan
Jul 25, 2021·International Journal of Molecular Sciences·Shraddha K DahaleSaikat Bhattacharjee
Aug 16, 2021·Trends in Plant Science·Mackenzie E W LorangerJacqueline Monaghan
Sep 28, 2021·Frontiers in Molecular Biosciences·Kai-Lu ZhangShao-Ming Zhou

❮ Previous
Next ❯

Methods Mentioned

BETA
immunoprecipitation
PCR
RNA-seq
two-hybrid
transgenic
coimmunoprecipitation
ChIP
RIP

Software Mentioned

REViGO

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.