Dynamic relationship of focal contacts and hemidesmosome protein complexes in live cells.

The Journal of Investigative Dermatology
Toshiyuki OzawaHiromi Kobayashi

Abstract

Epidermal cells adhere to the basement membrane zone through cell-matrix junctions termed hemidesmosomes. During wound healing, hemidesmosomes are disassembled to allow keratinocytes to move over wound sites. Such movement is mediated by both hemidesmosome protein complexes (HPCs) and focal contacts (FCs). In this study, we analyzed the interaction between HPCs and FCs in live HaCat cells expressing yellow fluorescent protein (YFP)-tagged beta4 integrin and cyan fluorescent protein (CFP)-tagged alpha-actinin as markers of HPCs and FCs, respectively. In HaCat cells migrating to repopulate wounds, FC proteins cluster rapidly in the direction of the wound. HPC assembly then follows and the newly formed HPCs occupy sites vacated by the disassembled FCs. HPC dynamics are dramatically reduced, and HaCat cells cease migration upon treatment with reagents that affect FC integrity/function. Upon treatment with reagents that destabilize HPCs, the dynamics of FCs in HaCat cells at the edges of wounds are enhanced, although FC assembly is irregular and the migration of the cells is aberrant. We also show that the complex interaction between hemidesmosomes and FCs in keratinocytes is myosin dependent and requires energy. In summary, we sugg...Continue Reading

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Citations

Jun 12, 2013·Histochemistry and Cell Biology·Maria J CastañónGerhard Wiche
Oct 23, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kevin J HamillJonathan C R Jones
Mar 29, 2014·Advances in Wound Care·Susan B HopkinsonJonathan C R Jones
Feb 2, 2011·Journal of Dermatological Science·Gilles LemaîtreGilles Waksman
Feb 14, 2012·The American Journal of Pathology·Haiyan QuMonique Aumailley
Aug 17, 2012·The Journal of Investigative Dermatology·Kristin SeltmannThomas M Magin
Nov 29, 2014·The Journal of Investigative Dermatology·Kevin J HamillJonathan C R Jones
May 14, 2010·The Journal of Investigative Dermatology·Yasuo Kitajima
May 29, 2015·Cell and Tissue Research·Gernot WalkoGerhard Wiche
Dec 10, 2014·Cell and Tissue Research·Gernot WalkoGerhard Wiche
Dec 3, 2014·Current Opinion in Cell Biology·Gerhard WicheMaria J Castañón
Jan 23, 2013·The American Journal of Pathology·Sho HiroyasuDaisuke Tsuruta
Jun 7, 2014·Medical Molecular Morphology·Toshiyuki OzawaDaisuke Tsuruta
Mar 23, 2011·Medical Molecular Morphology·Toshiyuki Ozawa, Daisuke Tsuruta
Jan 9, 2020·The Journal of Cell Biology·Wei WangArnoud Sonnenberg
Dec 29, 2019·Scientific Reports·Praachi B JainFlorence Janody
May 2, 2018·Cellular and Molecular Bioengineering·Hoda ZarkoobE A Sander
May 9, 2013·Experimental Dermatology·Hiroaki Iwata, Yasuo Kitajima
Jun 12, 2013·Journal of Cell Science·Jessica L EisenbergJonathan C R Jones
Jun 22, 2012·Journal of Cell Science·Emilie FaureMaxime Lehmann

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