Dynamic relationships among type IIa bacteriocins: temperature effects on antimicrobial activity and on structure of the C-terminal amphipathic alpha helix as a receptor-binding region

Biochemistry
Kamaljit KaurJohn C Vederas

Abstract

Dynamic aspects of structural relationships among class IIa bacteriocins, which are antimicrobial peptides from lactic acid bacteria (LAB), have been examined by use of circular dichroism (CD), molecular dynamics (MD) simulations, and activity testing. Pediocin PA-1 is a potent class IIa bacteriocin, which contains a second C-terminal disulfide bond in addition to the highly conserved N-terminal disulfide bond. A mutant of pediocin PA-1, ped[M31Nle], wherein the replacement of methionine by norleucine (Nle) gives enhanced stability toward aerobic oxidation, was synthesized by solid-phase peptide synthesis to study the activity of the peptide in relation to its structure. The secondary structural analysis from CD spectra of ped[M31Nle], carnobacteriocin B2 (cbn B2), and leucocin A (leuA) at different temperatures suggests that the alpha-helical region of these peptides is important for target recognition and activity. Using molecular modeling and dynamic simulations, complete models of pediocin PA-1, enterocin P, sakacin P, and curvacin A in 2,2,2-trifluoroethanol (TFE) were generated to compare structural relationships among this class of bacteriocins. Their high sequence similarity allows for the use of homology modeling techn...Continue Reading

Citations

Jun 17, 2008·Applied and Environmental Microbiology·Leah A Martin-VisscherJohn C Vederas
Feb 8, 2006·Applied and Environmental Microbiology·Tatsuya Tominaga, Yoshinori Hatakeyama
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Djamel DriderHervé Prévost
Apr 30, 2008·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Clarissa S Sit, John C Vederas
Jan 10, 2009·Microbial Cell Factories·Maria Papagianni, Sofia Anastasiadou
Dec 12, 2012·International Journal of Molecular Sciences·Yanhua CuiHongyu Cui
Dec 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·Paul D CotterR Paul Ross
Jan 22, 2013·Organic & Biomolecular Chemistry·Kaitlyn M TowleJohn C Vederas
Jan 18, 2016·Biochimica Et Biophysica Acta·Panagiota K KyriakouYiannis N Kaznessis
Nov 21, 2015·Journal of Agricultural and Food Chemistry·Sara ArbuluPablo E Hernández
Feb 13, 2008·Biochimica Et Biophysica Acta·Kamaljit KaurSahar Ahmed
Nov 18, 2008·Chemical Biology & Drug Design·Reem BeleidKamaljit Kaur
Jul 22, 2008·Chembiochem : a European Journal of Chemical Biology·Darren J DerksenJohn C Vederas
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May 16, 2013·Bioorganic & Medicinal Chemistry·Krishna Chaitanya BodapatiKamaljit Kaur
Feb 7, 2012·Bioorganic & Medicinal Chemistry·Rania SoudyKamaljit Kaur
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Aug 10, 2010·The Journal of Physical Chemistry. B·Wael SolimanKamaljit Kaur
Mar 1, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Hashem EtayashKamaljit Kaur

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