Dynamic responses of the glutathione system to acute oxidative stress in dystrophic mouse (mdx) muscles

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Roy W R DudleyBasil J Petrof

Abstract

The precise mechanisms underlying skeletal muscle damage in Duchenne muscular dystrophy (DMD) remain ill-defined. Functional ischemia during muscle activation, with subsequent reperfusion during rest, has been documented. Therefore, one possibility is the presence of increased oxidative stress. We applied a model of acute hindlimb ischemia/reperfusion (I/R) in mdx mice (genetic homolog of DMD) to evaluate dynamic in vivo responses of dystrophic muscles to this form of oxidative stress. Before the application of I/R, mdx muscles showed: 1) decreased levels of total glutathione (GSH) with an increased oxidized (GSSG)-to-reduced (GSH) glutathione ratio; 2) greater activity of the GSH-metabolizing enzymes glutathione peroxidase (GPx) and glutathione reductase; and 3) lower activity levels of NADP-linked isocitrate dehydrogenase (ICDH) and aconitase, two metabolic enzymes that are sensitive to inactivation by oxidative stress and also implicated in GSH regeneration. Interestingly, nondystrophic muscles subjected to I/R exhibited similar changes in total glutathione, GSSG/GSH, GPx, ICDH, and aconitase. In contrast, all of the above remained stable in mdx muscles subjected to I/R. Taken together, these results suggest that mdx muscles...Continue Reading

References

Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J Mårtensson, A Meister
Mar 15, 1980·The Biochemical Journal·A AndrésA Machado
Oct 1, 1993·Acta Physiologica Scandinavica·J M LawlerD S Criswell
Jan 1, 1994·Physiological Reviews·B P Yu
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·B J PetrofH L Sweeney
May 1, 1996·The Journal of Experimental Medicine·M R WeiserM C Carroll
May 5, 1998·Neuromuscular Disorders : NMD·T A RandoA Franco
Aug 5, 1998·The American Journal of Physiology·S PudupakkamR F Potter
Aug 26, 1998·Biochemical and Biophysical Research Communications·U AnderssonE A Newsholme
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·G D ThomasR G Victor
Jan 8, 1999·Journal of the Neurological Sciences·M H DisatnikT A Rando
Jan 28, 1999·Biochemical and Biophysical Research Communications·S M LeeJ W Park
Nov 24, 1999·Free Radical Biology & Medicine·O W Griffith
May 8, 2000·Muscle & Nerve·M H DisatnikT A Rando
Aug 30, 2000·Cardiovascular Research·N S DhallaN Makino
Oct 20, 2000·The Journal of Surgical Research·L GürkeM Heberer
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·M SanderR G Victor
Apr 3, 2001·The Journal of Biological Chemistry·A C Nulton-Persson, L I Szweda
Jan 25, 2002·Annual Review of Pharmacology and Toxicology·Neil Hogg
Apr 27, 2002·Annals of the New York Academy of Sciences·Michael B Reid, William J Durham
May 7, 2002·American Journal of Physical Medicine & Rehabilitation·T H MercerP F Naish
Sep 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Blandine ComteChristine Des Rosiers
Oct 4, 2002·Free Radical Biology & Medicine·Eun Sun YangJeen-Woo Park
Dec 31, 2002·Journal of Applied Physiology·Li ZhangJames R Urbaniak
Jun 7, 2003·American Journal of Physiology. Endocrinology and Metabolism·Martin K AngeleIrshad H Chaudry
Sep 25, 2003·Physiological Reviews·Derek M Yellon, James M Downey
May 8, 2004·Free Radical Research·Joon-Hyuck YangJeen-Woo Park
Jul 24, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Mohamed BenderdourChristine Des Rosiers
Oct 2, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Michael A MosesCho Y Pang

❮ Previous
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Citations

Oct 30, 2007·Journal of Toxicology and Environmental Health. Part a·J Grace WallenbornUrmila P Kodavanti
Aug 2, 2011·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Hatice TohmaPeter G Arthur
Mar 14, 2012·PloS One·Ana C CalvoRosario Osta
Dec 24, 2013·Journal of Molecular and Cellular Cardiology·Joshua J Martindale, Joseph M Metzger
Jan 6, 2016·Journal of Muscle Research and Cell Motility·Magdalena KozakowskaJozef Dulak
May 18, 2010·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Alexander SpassovChristiane Kunert-Keil
Sep 23, 2009·Journal of Molecular and Cellular Cardiology·Yan BurelleChristine Des Rosiers
Feb 23, 2012·Scientific Reports·April K MarroneHalyna R Shcherbata
Apr 14, 2011·Journal of Pineal Research·Youssef HibaouiOlivier M Dorchies
Mar 16, 2011·Muscle & Nerve·Chad D MarkertRobert W Grange
Dec 31, 2009·Phytotherapy Research : PTR·Helen N SaadaNoaman A Eltahawy
Aug 11, 2010·The American Journal of Pathology·Lara KhouzamiFrançoise Pecker
Jun 24, 2011·Free Radical Research·Ahmed F El-ShafeyPeter G Arthur
Mar 13, 2015·Biological Trace Element Research·Luis Henrique Rapucci MoraesElaine Minatel
May 17, 2008·International Journal of Occupational Medicine and Environmental Health·Béatrice MercierMichel Prost
Dec 3, 2014·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Tina Tinkara PeterneljJeff S Coombes
Mar 27, 2016·Journal of Neurochemistry·Balaraju SunithaMuchukunte Mukunda Srinivas Bharath
May 5, 2017·Human Molecular Genetics·Sara PetrilloEnrico Bertini
Apr 24, 2009·Pflügers Archiv : European journal of physiology·Vyacheslav M ShkrylNatalia Shirokova
May 21, 2009·American Journal of Physical Medicine & Rehabilitation·Nicholas P EvansRobert W Grange
Nov 25, 2014·Molecular and Cellular Biochemistry·Tina Tinkara PeterneljJeff S Coombes
Feb 20, 2021·Nature Reviews. Disease Primers·Dongsheng DuanAnnemieke Aartsma-Rus

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