Dynamic sieving capillary electrophoresis analysis of xylitol selenite-induced apoptosis in SMMC-7221 cells

Biotechnology Letters
Ming LeiChao Qi

Abstract

DNA ladder fragments, regarded as a biochemical hallmark of apoptosis, have been separated quickly and successfully by capillary electrophoresis. Inter-nucleosomal DNA fragmentations induced by xylitol selenite were determined for the first time, while hydroxypropylmethylcellulose (HPMC) was served as the sieving matrix in dynamic sieving capillary electrophoresis. The calibration curve (r(2) = 0.991) was established and multiples of two different nucleosomes (140 and 180 bp) were formed in the presence of xylitol selenite. Selenium compounds inhibited carcinogenesis in animal models, SMMC-7221 cells and several other cells by increasing apoptosis. The described method was useful in elucidating the anticancer activities of xylitol selenite and other selenium compounds, which was more effective to detect small fragments than slab gel electrophoresis.

References

Mar 29, 2000·Journal of Chromatography. a·M T ValenzuelaJ M Ruiz de Almodóvar
Feb 24, 2001·Life Sciences·S LiuY Hu
Apr 11, 2001·Mutation Research·K El-Bayoumy
Jun 10, 2008·Mini Reviews in Medicinal Chemistry·Rajesh Naithani

❮ Previous
Next ❯

Citations

Nov 7, 2013·International Journal of Molecular Sciences·Jie LiuXiao-Yan Xin
Mar 29, 2014·Analytical and Bioanalytical Chemistry·Zhiyong Yang, Jonathan V Sweedler
Jan 15, 2014·International Journal of Biological Macromolecules·Huiling KongGlyn O Phillips

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis