PMID: 6399815Jan 1, 1984Paper

Dynamic structure of biological and model membranes: analysis by optical anisotropy decay measurement

Advances in Biophysics
K KinositaA Ikegami

Abstract

Rotational Brownian motion of molecules in membranes can be "visualized" by time-resolved measurement of the decay of anisotropy of various flash-induced optical signals, such as fluorescence, phosphorescence, delayed fluorescence, transient absorption, or fluorescence depletion. The basic principles of the various forms of anisotropy measurement are illustrated in a unified manner. In organized structures such as membranes, rotational motion is restricted in angular range. Methods of analysis of observed optical anisotropy decays for the case of restricted rotation are described; the emphasis is laid on the separate estimation of the two important parameters, the range and rate, that characterize the restricted rotation. Practical aspects of the analytical procedures are also discussed. As an example of application, recent work from the authors' laboratory is reviewed: dynamic structures of lipid hydrocarbon chain region of membranes have been revealed by time-resolved fluorescence depolarization studies. A lipophilic fluorescent probe 1,6-diphenyl-1,3,5-hexatriene was incorporated in model and biological membranes of known compositions. The decay of fluorescence anisotropy indicated that the rod-shaped probe molecules wobbled...Continue Reading

Citations

Jan 1, 1985·The Journal of Membrane Biology·Y Kimura, A Ikegami
Jan 10, 2009·European Biophysics Journal : EBJ·Thomas Starke-Peterkovic, Ronald J Clarke
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H VogelG Jung
Mar 29, 2011·Cold Spring Harbor Perspectives in Biology·Frederick A Heberle, Gerald W Feigenson
Jul 24, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ryohei YasudaKazuhiko Kinosita
Jan 8, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Carlotta MarianecciMaria Carafa
Mar 15, 2011·Expert Opinion on Drug Metabolism & Toxicology·Dmitri R Davydov
Dec 21, 2006·Biochimica Et Biophysica Acta·Gaëlle Le GoffRonald J Clarke
Apr 3, 1990·Biochemistry·K M Musier-Forsyth, G G Hammes
May 28, 1985·Biochimica Et Biophysica Acta·C D StubbsD Phillips
Aug 11, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Gustavo ScanavachiRosangela Itri
Dec 26, 2001·Chemical Reviews·A MishraG B Behera

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