Dynamic Sumoylation of a Conserved Transcription Corepressor Prevents Persistent Inclusion Formation during Hyperosmotic Stress

PLoS Genetics
Michelle L OeserRichard G Gardner

Abstract

Cells are often exposed to physical or chemical stresses that can damage the structures of essential biomolecules. Stress-induced cellular damage can become deleterious if not managed appropriately. Rapid and adaptive responses to stresses are therefore crucial for cell survival. In eukaryotic cells, different stresses trigger post-translational modification of proteins with the small ubiquitin-like modifier SUMO. However, the specific regulatory roles of sumoylation in each stress response are not well understood. Here, we examined the sumoylation events that occur in budding yeast after exposure to hyperosmotic stress. We discovered by proteomic and biochemical analyses that hyperosmotic stress incurs the rapid and transient sumoylation of Cyc8 and Tup1, which together form a conserved transcription corepressor complex that regulates hundreds of genes. Gene expression and cell biological analyses revealed that sumoylation of each protein directs distinct outcomes. In particular, we discovered that Cyc8 sumoylation prevents the persistence of hyperosmotic stress-induced Cyc8-Tup1 inclusions, which involves a glutamine-rich prion domain in Cyc8. We propose that sumoylation protects against persistent inclusion formation during ...Continue Reading

References

Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M B EisenD Botstein
Dec 1, 1999·The Journal of Cell Biology·E S Johnson, G Blobel
Feb 1, 2003·Journal of Clinical Pathology·B KaplanM Pras
Sep 26, 2003·The Journal of Biological Chemistry·Dmitry S KryndushkinVitaly V Kushnirov
Nov 5, 2003·Molecular Biology of the Cell·Sean M O'Rourke, Ira Herskowitz
Feb 21, 2004·Bioinformatics·Robertson Craig, Ronald C Beavis
Jun 11, 2004·Annual Review of Biochemistry·Erica S Johnson
Jul 21, 2004·Journal of Structural and Functional Genomics·Michael P MalakhovTauseef R Butt
Aug 5, 2004·The Journal of Biological Chemistry·Vikram Govind PanseEd Hurt
Nov 16, 2004·Molecular & Cellular Proteomics : MCP·Carilee DenisonSteven P Gygi
Dec 14, 2004·The Journal of Biological Chemistry·J Thomas HannichMark Hochstrasser
Dec 15, 2004·Molecular & Cellular Proteomics : MCP·Dennis D Wykoff, Erin K O'Shea
Jul 19, 2005·Nature Biotechnology·Edda KlippStefan Hohmann
Dec 3, 2005·Protein Science : a Publication of the Protein Society·Jeffrey G MarblestoneTauseef R Butt
Jul 18, 2006·Nucleic Acids Research·Yu XueXuebiao Yao
Aug 2, 2007·Analytical and Bioanalytical Chemistry·Marcus BantscheffBernhard Kuster
Oct 4, 2007·Human Molecular Genetics·Jieya Shao, Marc I Diamond
Nov 15, 2007·Nature Reviews. Molecular Cell Biology·Ruth Geiss-Friedlander, Frauke Melchior
Jan 19, 2008·Biochemical and Biophysical Research Communications·Yoshifumi KobayashiTokichi Miyakawa
Nov 5, 2008·The Journal of Cell Biology·J Ross BuchanRoy Parker
Jan 14, 2009·Molecular and Cellular Biology·Zheng Wang, Gregory Prelich
Feb 17, 2009·Nature Cell Biology·Basant K PatelSusan W Liebman
May 28, 2009·Science Signaling·Filip GolebiowskiRonald T Hay
Jun 6, 2009·The Journal of Biological Chemistry·Sarmistha MukherjeeJorge A Iñiguez-Lluhí
Feb 26, 2010·International Journal of Cell Biology·Simone FuldaAfshin Samali
May 28, 2010·Genes & Development·Emanuel RosoninaJames L Manley
Sep 4, 2010·Proceedings of the National Academy of Sciences of the United States of America·Marcus J MillerRichard D Vierstra
Sep 14, 2010·Nature·Mario H Bengtson, Claudio A P Joazeiro
Oct 5, 2010·Advances in Experimental Medicine and Biology·Elise A KikisRichard I Morimoto
Jan 7, 2011·American Journal of Physiology. Cell Physiology·Kenneth W DunnJohn H McDonald
May 27, 2011·American Journal of Physiology. Cell Physiology·Kris BurkewitzKevin Strange
Jun 23, 2011·Science Signaling·Michael H TathamRonald T Hay
Jul 13, 2011·The Journal of Cell Biology·Petranka KrumovaJochen H Weishaupt

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Citations

Jan 18, 2017·Proceedings of the National Academy of Sciences of the United States of America·Pierre ChymkowitchJorrit M Enserink
Jun 24, 2016·American Journal of Physiology. Cell Physiology·Frauke Liebelt, Alfred C O Vertegaal
Mar 7, 2019·Journal of Cell Science·Hanne FolzCatherine Dargemont
Jul 10, 2019·Biology Open·Alexander I AlexandrovMichael O Agaphonov
May 10, 2020·Nature Communications·Janis Tam, Folkert J van Werven
Jun 6, 2020·Scientific Reports·Pratibha SiwachDaniel Kaganovich
Jan 8, 2021·Biomolecules·Dina FranićMirta Boban
Nov 13, 2020·Scientific Reports·Sundararaghavan PattabiramanDaniel Kaganovich
Apr 1, 2021·Molecular Biology of the Cell·Amanda I BradleyRichard G Gardner

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Datasets Mentioned

BETA
GSE57476

Methods Mentioned

BETA
sumoylation
protein folding
pulldown
fluorescence microscopy
Structured
fluorence microscopy
confocal microscopy
electrophoresis
PCR

Software Mentioned

! Tandem
Agilent
Peptide Prophet
Photoshop CS
Elements
NIS

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