PMID: 11906917Mar 22, 2002Paper

Dynamic testicular adhesion junctions are immunologically unique. I. Localization of p120 catenin in rat testis

Biology of Reproduction
Kamin J Johnson, Kim Boekelheide

Abstract

In the seminiferous epithelium, morphologically diverse junctions mediate inter-Sertoli and Sertoli-germ cell adhesive contact, but the molecular composition of such junctions is not well known. At prototypical adherens junctions, proteins termed catenins bind to the intracellular domain of classic cadherins and regulate the strength of adhesion. Using a panel of monoclonal antibodies (5A7, 8D11, and 15D2), p120 catenin (p120) was localized in postnatal and adult rat testis cryosections and touch preparations by immunofluorescence. Immunoprecipitation of testis homogenates showed that at least four p120 isoforms were expressed from Postnatal Day 7 through adulthood. Both inter-Sertoli and Sertoli-germ cell junctions were p120-positive, however, individual p120 monoclonals were localized to specific junctions. The 5A7 and 8D11 antibodies colocalized with beta-catenin and plectin at inter-Sertoli and Sertoli-spermatocyte junctions. At inter-Sertoli junctions, p120 was juxtaposed to but did not colocalize with f-actin. Thus, p120 is likely a component of inter-Sertoli desmosome-like junctions. In contrast, the 15D2 monoclonal antibody specifically immunostained Sertoli-round spermatid and inter-Sertoli cell junctions in a dynamic ...Continue Reading

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Citations

Jul 26, 2005·Histochemistry and Cell Biology·Nobuo TeradaShinichi Ohno
Apr 18, 2003·Biology of Reproduction·Yong-mei ChenC Yan Cheng
Oct 3, 2003·Biology of Reproduction·Kamin J JohnsonEun J Kwon
Apr 2, 2004·Biology of Reproduction·Nikki P Y Lee, C Yan Cheng
Oct 22, 2004·Biology of Reproduction·Reza J Rasoulpour, Kim Boekelheide
Feb 19, 2010·Journal of Cell Science·Pierre D McCrea, Dongmin Gu
Oct 29, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Takeshi SatoYoshihiro Hayashi
Sep 3, 2009·Microscopy Research and Technique·María Leiza VitaleR-Marc Pelletier
Feb 15, 2007·International Journal of Andrology·K PospechovaV Semecky
May 9, 2006·Reproductive Toxicology·Cristian M SobarzoBerta Denduchis
May 21, 2013·Spermatogenesis·Cecilia V PérezLivia Lustig
May 20, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Nobuo TeradaShinichi Ohno
Sep 7, 2006·Molecular Reproduction and Development·Marina PriscoPiero Andreuccetti

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