Dynamical Regulation of Enzyme Cascade Amplification by a Regenerated DNA Nanotweezer for Ultrasensitive Electrochemical DNA Detection
Abstract
Traditional scaffolds such as metal nanoparticles and DNA origami remain a considerable challenge to regulate the enzyme cascade catalytic efficiency dynamically and reversibly on account of their irreversible conformation. To address these issues, a regenerated DNA tweezer was designed to dynamically regulate the interenzyme spacing for high-efficiency enzyme cascade amplification for homogeneous determination of target DNA related to cancer diseases. Initially, the enzyme-functionalized DNA tweezer was maintained at the opened state with a relatively distant interenzyme distance (19-24 nm), leading to a low catalytic efficiency. Benefiting from target induced Mg2+-dependent DNAzyme cleavage recycling, the one input target could be transduced to multiple corresponding methylene blue (MB) labeled DNA (S5), which served not only as the signal probe to provide a detectable electrochemical signal but also fuel to switch the DNA tweezer from the opened to closed state, leading to cascaded enzymes close enough (5-10 nm) for enhancing the catalytic efficiency for sensitive target DNA analysis with a low detection limit down to 30 fM. In the presence of antifuels, the closed DNA tweezer easily switched back to the opened state via a o...Continue Reading
References
Rational Engineering of a Dynamic, Entropy-Driven DNA Nanomachine for Intracellular MicroRNA Imaging
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