Dynamically Long-Term Imaging of Cellular RNA by Fluorescent Carbon Dots with Surface Isoquinoline Moieties and Amines

Analytical Chemistry
Yunying ChengCheng Zhi Huang

Abstract

Cellular RNA dynamics are closely associated with a vast range of physiological processes that are mostly long-lasting. To uncover the association between RNA dynamics and these processes, fluorescent RNA probes with high specificity, photostability, and biocompatibility are compulsory. Herein, a series of fluorescent carbon dots (CDs) have been prepared by one-pot hydrothermal treatment of o-, m-, or p-phenylenediamines with triethylenetetramine. Only CDs derived from the meta precursor ( m-CDs) with excellent photostability and biocompatibility can specifically bind to cellular RNA, allowing successfully long-term (up to 3 days) monitoring of RNA dynamics during cell apoptosis, mitosis, and proliferation. This RNA affinity can be attributed to the isoquinoline moieties and amines on the surface of m-CDs, which can bind to RNA through π-π stacking and electrostatic bonding, respectively. The cellular internalization of m-CDs is time-, temperature-, ATP-, caveolar, and microtubule-dependent. Additionally, investigations on the in vivo behavior of m-CD suggest that they can be efficiently and rapidly excreted from the zebrafish larvae body after 48 h. Our results provide a powerful tool for clarifying complex relationships betwe...Continue Reading

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Citations

Feb 20, 2019·Small·Jianjun DuXiaojun Peng
Feb 11, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Haydar AliNikhil R Jana
Mar 24, 2020·Luminescence : the Journal of Biological and Chemical Luminescence·Zhu HanXiaoli Hu
Aug 12, 2020·Journal of Fluorescence·Yingying HuQiaoli Yue
Aug 26, 2020·Chemistry : a European Journal·Zhiming HuoXiaohua Xiao

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