Dynamics and mechanism of light harvesting in UV photoreceptor UVR8.

Chemical Science
Xiankun LiDongping Zhong

Abstract

Photosynthetic pigments form light-harvesting networks to enable nearly perfect quantum efficiency in photosynthesis via excitation energy transfer. However, similar light-harvesting mechanisms have not been reported in light sensing processes in other classes of photoreceptors during light-mediated signaling. Here, based on our earlier report, we mapped out a striking energy-transfer network composed of 26 structural tryptophan residues in the plant UV-B photoreceptor UVR8. The spectra of the tryptophan chromophores are tuned by the protein environments, funneling all excitation energy to a cluster of four tryptophan residues, a pyramid center, where the excitation-induced monomerization is initiated for cell signaling. With extensive site-directed mutagenesis, various time-resolved fluorescence techniques, and combined QM/MM simulations, we determined the energy-transfer rates for all donor-acceptor pairs, revealing the time scales from tens of picoseconds to nanoseconds. The overall light harvesting quantum efficiency by the pyramid center is significantly increased to 73%, compared to a direct excitation probability of 35%. UVR8 is the only photoreceptor discovered so far using a natural amino-acid tryptophan without utiliz...Continue Reading

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Citations

Feb 28, 2021·Annual Review of Plant Biology·Roman PodolecRoman Ulm

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Methods Mentioned

BETA
X-ray
light harvesting
fluorescence spectroscopy
Fluorescence
FRET
the

Software Mentioned

GROMACS
CHARMM
FluoFit
GAMESS

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