Dynamics in the transient complex of plastocyanin-cytochrome f from Prochlorothrix hollandica

Journal of the American Chemical Society
Rinske HulskerMarcellus Ubbink

Abstract

The nature of transient protein complexes can range from a highly dynamic ensemble of orientations to a single well-defined state. This represents variation in the equilibrium between the encounter and final, functional state. The transient complex between plastocyanin (Pc) and cytochrome f (cytf) of the cyanobacterium Prochlorothrix hollandica was characterized by NMR spectroscopy. Intermolecular pseudocontact shifts and chemical shift perturbations were used as restraints in docking calculations to determine the structure of the wild-type Pc-cytf complex. The orientation of Pc is similar to orientations found in Pc-cytf complexes from other sources. Electrostatics seems to play a modest role in complex formation. A large variability in the ensemble of lowest energy structures indicates a dynamic nature of the complex. Two unusual hydrophobic patch residues in Pc have been mutated to the residues found in other plastocyanins (Y12G/P14L). The binding constants are similar for the complexes of cytf with wild-type Pc and mutant Pc, but the chemical shift perturbations are smaller for the complex with mutant Pc. Docking calculations for the Y12G/P14L Pc-cytf complex did not produce a converged ensemble of structures. Simulations o...Continue Reading

References

Jan 1, 1989·Journal of Biochemistry·T Takabe, H Ishikawa
Dec 5, 1993·Journal of Molecular Biology·A Sali, T L Blundell
Oct 25, 2001·Journal of the American Chemical Society·P B CrowleyM Ubbink
Jun 20, 2002·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Zhao-Xun LiangBrian M Hoffman
Feb 5, 2003·Journal of Magnetic Resonance·Charles D SchwietersG Marius Clore
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·W. A. CramerJ. L. Smith
Dec 2, 2004·Nature Reviews. Molecular Cell Biology·Nathan Nelson, Adam Ben-Shem
Feb 4, 2005·Protein Science : a Publication of the Protein Society·Alexander N VolkovMarcellus Ubbink
Dec 6, 2005·Photosynthesis Research·Derek S Bendall
May 6, 2006·Quarterly Reviews of Biophysics·Fabio ArnesanoMario Piccioli
Dec 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·Alexander N VolkovMarcellus Ubbink

❮ Previous
Next ❯

Citations

Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Young C KimGerhard Hummer
Mar 12, 2009·FEBS Letters·Marcellus Ubbink
Mar 1, 2011·The FEBS Journal·Qamar BashirMarcellus Ubbink
May 20, 2009·Biophysical Journal·Michal HarelGideon Schreiber
Sep 12, 2015·Journal of the American Chemical Society·Cedric P OwensF Akif Tezcan
Jun 6, 2015·Journal of Biomolecular NMR·Witold AndrałojćLuca Sgheri
Aug 13, 2013·Current Opinion in Structural Biology·Jesika Schilder, Marcellus Ubbink
Nov 25, 2014·Journal of Biomolecular NMR·Mauro RinaldelliClaudio Luchinat
Aug 22, 2014·The Journal of Physical Chemistry. B·Witold AndrałojćEnrico Ravera
Feb 8, 2014·Chembiochem : a European Journal of Chemical Biology·Jia-Ying GuanMarcellus Ubbink
Aug 13, 2013·Physical Chemistry Chemical Physics : PCCP·Suwipa Saen-OonVictor Guallar
May 24, 2012·Chembiochem : a European Journal of Chemical Biology·Sandra ScanuMarcellus Ubbink
May 10, 2019·Journal of Molecular Biology·Madeleine StricklandNico Tjandra
Mar 12, 2017·Progress in Nuclear Magnetic Resonance Spectroscopy·Christoph Nitsche, Gottfried Otting
Aug 3, 2010·Journal of the American Chemical Society·Alexander N VolkovMarcellus Ubbink

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.