Dynamics of anterior-posterior axis formation in the developing mouse embryo.

Nature Communications
Samantha A MorrisMagdalena Zernicka-Goetz

Abstract

The development of an anterior-posterior (AP) polarity is a crucial process that in the mouse has been very difficult to analyse, because it takes place as the embryo implants within the mother. To overcome this obstacle, we have established an in-vitro culture system that allows us to follow the step-wise development of anterior visceral endoderm (AVE), critical for establishing AP polarity. Here we use this system to show that the AVE originates in the implanting blastocyst, but that additional cells subsequently acquire AVE characteristics. These 'older' and 'younger' AVE domains coalesce as the egg cylinder emerges from the blastocyst structure. Importantly, we show that AVE migration is led by cells expressing the highest levels of AVE marker, highlighting that asymmetry within the AVE domain dictates the direction of its migration. Ablation of such leading cells prevents AVE migration, suggesting that these cells are important for correct establishment of the AP axis.

References

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Citations

May 31, 2014·Nature Communications·Yeh-Chuin PohNing Wang
Oct 31, 2014·Nature Protocols·Ivan BedzhovMagdalena Zernicka-Goetz
Oct 29, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Ivan BedzhovMagdalena Zernicka-Goetz
Oct 29, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Costis Papanayotou, Jérôme Collignon
Oct 18, 2015·Seminars in Cell & Developmental Biology·Hiroshi Hamada
Jun 4, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Mary C WallingfordJesse Mager
Mar 27, 2013·Reproductive Biomedicine Online·Agnieszka Jedrusik
Dec 10, 2013·Stem Cell Reports·Clair E WeidgangAlexander Kleger
May 5, 2016·Nature Cell Biology·Marta N ShahbaziMagdalena Zernicka-Goetz
Jul 13, 2016·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Idse Heemskerk, Aryeh Warmflash
Oct 5, 2016·Mechanisms of Development·Isao Matsuo, Ryuji Hiramatsu
Nov 20, 2016·Molecular Reproduction and Development·Yasuhisa MunakataHisataka Iwata
Jan 27, 2017·Open Biology·Samantha A Morris
Mar 16, 2017·Development·Mijo Simunovic, Ali H Brivanlou
Aug 11, 2018·Annual Review of Cell and Developmental Biology·Hui Ting Zhang, Takashi Hiiragi
Apr 3, 2019·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Nicole A Theodosiou, Emmanuela Oppong
Jan 10, 2020·Cell Proliferation·Zhen GuQi Gu
Aug 25, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Néstor Saiz, Anna-Katerina Hadjantonakis
Jul 25, 2018·Nature Cell Biology·Marta N Shahbazi, Magdalena Zernicka-Goetz
Jul 11, 2018·Nature Protocols·Sarah Ellys HarrisonMagdalena Zernicka-Goetz
Jan 8, 2021·Human Reproduction Update·Samuel OjosnegrosAnna Veiga
Jan 30, 2021·Reproductive Sciences·Emilio Francés-HerreroIrene Cervelló
Feb 7, 2021·Development·Lillian B SpatzJason C Mills
Feb 12, 2021·Development, Growth & Differentiation·Katsunori Semi, Yasuhiro Takashima
Sep 25, 2021·Environmental Science and Pollution Research International·Zhihua ZhaoYang Yang

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Methods Mentioned

BETA
transgenic
confocal microscopy

Software Mentioned

Slidebook
Image J
ImageJ
Adobe Photoshop

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