Dynamics of Aspen Roots Colonization by Pseudomonads Reveals Strain-Specific and Mycorrhizal-Specific Patterns of Biofilm Formation

Frontiers in Microbiology
Marie-Francoise Noirot-GrosPhilippe H Noirot

Abstract

Rhizosphere-associated Pseudomonas fluorescens are known plant growth promoting (PGP) and mycorrhizal helper bacteria (MHB) of many plants and ectomycorrhizal fungi. We investigated the spatial and temporal dynamics of colonization of mycorrhizal and non-mycorrhizal Aspen seedlings roots by the P. fluorescens strains SBW25, WH6, Pf0-1, and the P. protegens strain Pf-5. Seedlings were grown in laboratory vertical plates systems, inoculated with a fluorescently labeled Pseudomonas strain, and root colonization was monitored over a period of 5 weeks. We observed unexpected diversity of bacterial assemblies on seedling roots that changed over time and were strongly affected by root mycorrhization. P. fluorescens SBW25 and WH6 stains developed highly structured biofilms with internal void spaces forming channels. On mycorrhizal roots bacteria appeared encased in a mucilaginous substance in which they aligned side by side in parallel arrangements. The different phenotypic classes of bacterial assemblies observed for the four Pseudomonas strains were summarized in a single model describing transitions between phenotypic classes. Our findings also reveal that bacterial assembly phenotypes are driven by interactions with mucilaginous ma...Continue Reading

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Citations

Mar 17, 2019·Microorganisms·Oyungerel NatsagdorjTakuji Ohwada
Feb 1, 2020·Journal of the Science of Food and Agriculture·Alejandro Jiménez-GómezRaúl Rivas
Feb 9, 2020·Journal of Applied Microbiology·A PanditM Kochar
Oct 2, 2020·Microbial Biotechnology·Laura RieussetClaire Prigent-Combaret
Nov 7, 2019·Scientific Reports·Marie-Francoise Noirot-GrosPhilippe Noirot
Nov 6, 2020·FEMS Microbiology Ecology·Thabiso E MotaungEmma Steenkamp
Nov 12, 2020·FEMS Microbiology Letters·Federico Rossi

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Methods Mentioned

BETA
PCR
fluorescence microscopy
confocal microcopy
transmission electron microscopy

Software Mentioned

NIS
Elements Imaging
Cytoscape

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