Dynamics of bacterial community in solid-state fermented feed revealed by 16S rRNA

Letters in Applied Microbiology
Z YuW Lu


The aim of the present study was to monitor the changes in the composition of microbiota in solid-state fermented feed and to evaluate their biosafety. In the solid-state fermentation, six probiotic bacteria strains were used as inoculum and soybean meal were used as carbohydrate source. At 0, 1, 2, 3, 5, 7, 10 and 15 days, samples were collected for further analysis. Denaturing gradient gel electrophoresis (DGGE) analysis showed that Bifidobacterium bifidum and Bacillus licheniformis were always present throughout the fermentation. Bifidobacterium bifidum, Enterococcus faecalis and Lactobacillus acidophilum were dominant throughout the entire fermentation as monitored by Lactobacillus-specific PCR-DGGE. Probiotics supplementation could reduce the levels of the pathogenic bacteria such as Staphylococcus aureus and enterotoxigenic Escherichia coli (ETEC) by species-specific real-time PCR. And Salmonella spp. was not detected throughout the entire fermentation. Probiotics supplemented are always dominant throughout the whole period of solid-state fermentation and effective in preventing the growth of pathogens. Based on the results, the high quality, stable solid-state fermented feed could be produced and applied in the pigs to i...Continue Reading


Apr 1, 1991·Clinics in Dermatology·R P Rapini
Sep 1, 1990·FEMS Microbiology Reviews·S E Lindgren, W J Dobrogosz
Nov 5, 1999·Applied and Environmental Microbiology·Y Nebra, A R Blanch
Oct 31, 2000·Applied and Environmental Microbiology·M T SuzukiE F DeLong
Jun 27, 2001·Applied and Environmental Microbiology·R L van WinsenF van Knapen
Sep 14, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·John A CrumpFrederick J Angulo
Jan 7, 2003·Applied and Environmental Microbiology·Burkhard MalornyReiner Helmuth
Feb 1, 1963·The American Journal of Medicine·S BRUNJESV J JOHNS

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