Dynamics of brassinosteroid response modulated by negative regulator LIC in rice.

PLoS Genetics
Cui ZhangKang Chong

Abstract

Brassinosteroids (BRs) regulate rice plant architecture, including leaf bending, which affects grain yield. Although BR signaling has been investigated in Arabidopsis thaliana, the components negatively regulating this pathway are less well understood. Here, we demonstrate that Oryza sativa LEAF and TILLER ANGLE INCREASED CONTROLLER (LIC) acts as an antagonistic transcription factor of BRASSINAZOLE-RESISTANT 1 (BZR1) to attenuate the BR signaling pathway. The gain-of-function mutant lic-1 and LIC-overexpressing lines showed erect leaves, similar to BZR1-depleted lines, which indicates the opposite roles of LIC and BZR1 in regulating leaf bending. Quantitative PCR revealed LIC transcription rapidly induced by BR treatment. Image analysis and immunoblotting showed that upon BR treatment LIC proteins translocate from the cytoplasm to the nucleus in a phosphorylation-dependent fashion. Phosphorylation assay in vitro revealed LIC phosphorylated by GSK3-like kinases. For negative feedback, LIC bound to the core element CTCGC in the BZR1 promoter on gel-shift and chromatin immunoprecipitation assay and repressed its transcription on transient transformation assay. LIC directly regulated target genes such as INCREASED LEAF INCLINATION ...Continue Reading

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Citations

Mar 29, 2014·Plant Cell Reports·Cui ZhangKang Chong
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Methods Mentioned

BETA
immunoprecipitation
PCR
ChIP
Gel shift assay
two-hybrid
transgenic
Gel
transfection
Gel shift
Kinase

Software Mentioned

MEME
BLAST
MetaMorph
ImageJ
MEGA

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