DOI: 10.1101/503730Dec 21, 2018Paper

Dynamics of centriole amplification in centrosome-depleted brain multiciliated progenitors

BioRxiv : the Preprint Server for Biology
Olivier MERCEYAlice Meunier

Abstract

Centrioles are essential microtubule-based organelles organizing cilia and centrosomes. Their mode of biogenesis is semi-conservative: each pre-existing centriole scaffolds the formation of a new one, a process coordinated with the cell cycle. By contrast, multiciliated progenitors with two centrosomal centrioles massively amplify centrioles to support the nucleation of hundred of motile cilia and transport vital fluids. This occurs through cell type-specific organelles called deuterosomes, composed of centrosome-related elements, and is regulated by the cell cycle machinery. Deuterosome-dependent centriole amplification was proposed for decades to occur de novo, i.e. independently from pre-existing centrioles. Challenging this hypothesis, we recently reported an accumulation of procentriole and deuterosome precursors at the centrosomal daughter centriole during centriole amplification in brain multiciliated cells. Here we further investigate the relationship between the centrosome and the dynamic of centriole amplification by (i) characterizing the centrosome behavior during the centriole amplification dynamics and (ii) assessing the dynamics of amplification in centrosome-depleted cells. Surprisingly, although our data streng...Continue Reading

Related Concepts

Cell Cycle
Cilia
Microtubules
Stem Cells
Focal
Anabolism
Centrosome
Brain Cell
Carcinogenesis
DNA Amplification

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