Dynamics of Co-translational Membrane Protein Integration and Translocation via the Sec Translocon.

Journal of the American Chemical Society
Michiel J M NiesenThomas F Miller

Abstract

An important aspect of cellular function is the correct targeting and delivery of newly synthesized proteins. Central to this task is the machinery of the Sec translocon, a transmembrane channel that is involved in both the translocation of nascent proteins across cell membranes and the integration of proteins into the membrane. Considerable experimental and computational effort has focused on the Sec translocon and its role in nascent protein biosynthesis, including the correct folding and expression of integral membrane proteins. However, the use of molecular simulation methods to explore Sec-facilitated protein biosynthesis is hindered by the large system sizes and long (i.e., minute) time scales involved. In this work, we describe the development and application of a coarse-grained simulation approach that addresses these challenges and allows for direct comparison with both in vivo and in vitro experiments. The method reproduces a wide range of experimental observations, providing new insights into the underlying molecular mechanisms, predictions for new experiments, and a strategy for the rational enhancement of membrane protein expression levels.

References

Oct 1, 1996·Nature Structural Biology·W C Wimley, S H White
Aug 19, 2000·Nature·J C SamuelsonR E Dalbey
Sep 5, 2001·FEBS Letters·V Goder, M Spiess
Jul 11, 2003·The EMBO Journal·Veit Goder, Martin Spiess
Nov 25, 2003·FEBS Letters·Donald M EngelmanJean-Luc Popot
Dec 9, 2003·Nature·Bert Van den BergTom A Rapoport
Nov 20, 2004·The Journal of Chemical Physics·Serdal KirmizialtinDmitrii E Makarov
Dec 2, 2005·Nature·James U Bowie
Dec 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Guy ZivD Thirumalai
Mar 11, 2006·Biotechnology and Bioengineering·Lydia M Contreras MartínezMatthew P DeLisa
Jul 6, 2006·Trends in Biotechnology·Samuel WagnerJan-Willem de Gier
Oct 25, 2006·Biochemistry·Shozeb HaiderMark S P Sansom
Dec 2, 2006·Nature Reviews. Drug Discovery·John P OveringtonAndrew L Hopkins
Dec 2, 2006·Nature Reviews. Molecular Cell Biology·Gunnar von Heijne
Sep 1, 2007·Biochemistry·James Gumbart, Klaus Schulten
Feb 5, 2008·Journal of Molecular Biology·Oded LewinsonDouglas C Rees
Jun 25, 2008·Annual Review of Biophysics·Stephen H White, Gunnar von Heijne
Sep 27, 2008·Nano Letters·Edward P O'BrienBernard R Brooks
Jun 6, 2009·Annual Review of Biochemistry·William Dowhan, Mikhail Bogdanov
Oct 28, 2009·Journal of Structural and Functional Genomics·Marco PuntaBurkhard Rost
Nov 26, 2009·Journal of Molecular Biology·Linnea E HedinArne Elofsson
Feb 2, 2010·Biochemical and Biophysical Research Communications·Koreaki ItoKit Pogliano
Mar 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Bin Zhang, Thomas F Miller
May 29, 2010·Science·Susanna SeppäläGunnar von Heijne
Sep 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Anna RychkovaArieh Warshel
Nov 11, 2010·Journal of the American Chemical Society·Edward P O'BrienChristopher M Dobson
Feb 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·James GumbartKlaus Schulten
Apr 22, 2011·Biotechnology Journal·Rhodri SaundersCharlotte M Deane
May 25, 2011·Proceedings of the National Academy of Sciences of the United States of America·C Preston Moon, Karen G Fleming
May 25, 2011·Proceedings of the National Academy of Sciences of the United States of America·Karin ÖjemalmGunnar von Heijne
Sep 3, 2011·Biochimica Et Biophysica Acta·Morten H H NørholmDaniel O Daley
Aug 3, 2012·Journal of the American Chemical Society·Bin Zhang, Thomas F Miller
Sep 25, 2012·Nature Structural & Molecular Biology·Nurzian IsmailGunnar von Heijne
Oct 23, 2012·Cell Reports·Bin Zhang, Thomas F Miller
Dec 12, 2012·The Journal of Cell Biology·Steven F TruemanReid Gilmore
Dec 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Prajwal CiryamEdward P O'Brien
Dec 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Anna Rychkova, Arieh Warshel
Jun 5, 2013·Proceedings of the National Academy of Sciences of the United States of America·Anna RychkovaArieh Warshel
Nov 6, 2013·Proceedings of the National Academy of Sciences of the United States of America·Erhan DemirciMartin Spiess

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biochimica Et Biophysica Acta
David J F du PlessisArnold J M Driessen
Seikagaku. The Journal of Japanese Biochemical Society
Tomoya Tsukazaki
© 2021 Meta ULC. All rights reserved