Dynamics of cohesin proteins REC8, STAG3, SMC1 beta and SMC3 are consistent with a role in sister chromatid cohesion during meiosis in human oocytes

Human Reproduction
R Garcia-CruzM Garcia Caldés

Abstract

Sister chromatid cohesion is essential for ordered chromosome segregation at mitosis and meiosis. This is carried out by cohesin complexes, comprising four proteins, which seem to form a ring-like complex. Data from animal models suggest that loss of sister chromatid cohesion may be involved in age-related non-disjunction in human oocytes. Here, we describe the distribution of cohesins throughout meiosis in human oocytes. We used immunofluorescence in human oocytes at different meiotic stages to detect cohesin subunits REC8, STAG3, SMC1 beta and SMC3, [also synaptonemal complex (SC) protein 3 and shugoshin 1]. Samples from euploid fetuses and adult women were collected, and 51 metaphase I (MI) and 113 metaphase II (MII) oocytes analyzed. SMC1 beta transcript levels were quantified in 85 maturing germinal vesicle (GV) oocytes from 34 women aged 19-43 years by real-time PCR. At prophase I, cohesin subunits REC8, STAG3, SMC1 beta and SMC3 overlapped with the lateral element of the SC. Short cohesin fibers are observed in the oocyte nucleus during dictyate arrest. All four subunits are observed at centromeres and along chromosomal arms, except at chiasmata, at MI and are present at centromeric domains from anaphase I to MII. SMC1 b...Continue Reading

References

Jan 1, 1996·Human Molecular Genetics·K E KoehlerT Hassold
Mar 4, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·N PezziJ L Barbero
Sep 1, 2000·Chromosoma·R Hancock
Jan 11, 2001·Chromosoma·J Wolstenholme, R R Angell
Apr 3, 2001·Nature Reviews. Genetics·T Hassold, P Hunt
Aug 3, 2001·Nature Cell Biology·I PrietoJ L Barbero
Sep 21, 2001·Molecular and Cellular Biology·E RevenkovaR Jessberger
May 2, 2002·Molecular Cell·Christian H HaeringKim Nasmyth
May 30, 2002·EMBO Reports·Ignacio PrietoJose L Barbero
Feb 5, 2003·Microscopy Research and Technique·I Martínez-FloresM Garcia
Jun 18, 2003·Human Genetics·Christian OlesenNiels Tommerup
Feb 5, 2004·Reproductive Biomedicine Online·U Eichenlaub-RitterR Gosden
May 6, 2004·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Ignacio PrietoJosé L Barbero
Sep 30, 2005·Cytogenetic and Genome Research·N E LambT J Hassold
Oct 19, 2005·Current Opinion in Cell Biology·Yoshinori Watanabe
Mar 17, 2006·Nature·Tomoya S KitajimaYoshinori Watanabe
Dec 18, 2007·Nature Cell Biology·Jibak LeeYoshinori Watanabe
Feb 7, 2008·Reproductive Biomedicine Online·Lin Liu, David L Keefe
Oct 25, 2008·Genome Dynamics·J A Suja, J L Barbero
Nov 15, 2008·PLoS Genetics·Vijayalakshmi V Subramanian, Sharon E Bickel

❮ Previous
Next ❯

Citations

Feb 10, 2012·European Journal of Human Genetics : EJHG·Alan H HandysideJoep Geraedts
Jun 19, 2012·Nature Reviews. Genetics·So I NagaokaPatricia A Hunt
Oct 1, 2011·Biology of Reproduction·Teresa ChiangMichael A Lampson
Dec 3, 2010·Genes & Development·Rolf Jessberger
May 9, 2014·PloS One·Makiko TsutsumiHiroki Kurahashi
Mar 3, 2012·Yi chuan = Hereditas·Wen-Jun XieWei-Wei Jin
Mar 7, 2014·The New England Journal of Medicine·Sandrine CaburetEric Vilain
Jan 1, 2014·Journal of Clinical Medicine·Maj A HulténJon Jonasson
Jun 22, 2014·ELife·Johanna Liinamaria SyrjänenOwen Richard Davies
Jun 27, 2015·Nature Genetics·Miguel A Brieño-Enríquez, Paula E Cohen
Mar 12, 2015·Journal of Assisted Reproduction and Genetics·Jessica E HornickTeresa K Woodruff
Jul 25, 2017·International Journal of Molecular Sciences·Jin-Mei Cheng, Yi-Xun Liu
Sep 21, 2011·Genes·Kim HollowayPaula E Cohen
Jan 1, 2010·Genes·Ekaterina RevenkovaRolf Jessberger
Jan 29, 2011·Cytogenetic and Genome Research·E FragouliJ D A Delhanty
Jan 22, 2011·Cytogenetic and Genome Research·J L Barbero
Jan 14, 2011·Cytogenetic and Genome Research·F Pacchierotti, U Eichenlaub-Ritter
Jun 14, 2016·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Wei MaAndreas Houben
Nov 28, 2018·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kei-Ichiro Ishiguro
May 12, 2019·Biology of Reproduction·Svetlana A Yatsenko, Aleksandar Rajkovic
Mar 31, 2015·The FEBS Journal·Emily L Kurdzo, Dean S Dawson
Feb 22, 2012·Congenital Anomalies·Hiroki KurahashiTamae Ohye
Feb 28, 2015·Aging Cell·Evelyne SaadeVasily Ogryzko
Jul 25, 2012·Aging Cell·Francesca E DuncanTeresa K Woodruff
Dec 16, 2017·Human Reproduction Update·Jessica GreaneyHayden Homer
Jan 18, 2018·Biology of Reproduction·Suk Namgoong, Nam-Hyung Kim
Apr 19, 2018·Chinese Journal of Integrative Medicine·Bin ZhangLing Wang
Nov 23, 2018·The Journal of Reproduction and Development·Gaku ShimoiYuichi Kameyama

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