PMID: 8967435May 1, 1996Paper

Dynamics of cortical granule exocytosis at fertilization in living mouse eggs

The American Journal of Physiology
M TaharaA Miyake

Abstract

Sperm-egg fusion induces an intracellular free calcium concentration ([Ca2+]i) increase and exocytosis of cortical granules (CGs). Recently we used an impermeable fluorescent membrane probe, 1-[4-(trimethylammonio)phenyl]-6-phenyl-1,3,5-hexatriene (TMA-DPH), to develop a method to evaluate the kinetics of exocytosis in single living cells. In this study we used digital imaging and confocal laser scanning microscopy to evaluate CG exocytosis in living mouse eggs with TMA-DPH. Time-related changes of CG exocytosis were estimated as the percent increase of TMA-DPH fluorescence. The increase of fluorescence in the egg started after sperm attachment, continued at an almost uniform rate, and ceased at 45-60 min. Whereas the [Ca2+]i increase at fertilization was transient or oscillatory, exocytosis was not always induced concomitantly with each [Ca2+]i peak. Next we used this method to determine some intracellular mediators of exocytosis in the egg. An intracellular calcium chelator, 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid-acetoxymethyl ester, and a microfilament inhibitor, cytochalasin B, blocked sperm-induced exocytosis. A guanosine 5'-triphosphate-binding protein activator, AlF4-, induced exocytosis. These results ...Continue Reading

Citations

Jun 5, 1998·The American Journal of Physiology·Y IkebuchiY Murata
Feb 12, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Vanessa L Horner, Mariana F Wolfner
May 11, 2013·Molecules and Cells·Hyoeun KangJeong Su Oh
Dec 24, 2005·Physiological Reviews·Michael Whitaker
Jul 27, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·John ParringtonGary Wessel
May 14, 2016·Reproductive Biology and Endocrinology : RB&E·Inmaculada MolinaEdurne Novella-Maestre
Aug 9, 2006·Reproductive Medicine and Biology·Hiroyuki Suzuki, Yosuke Saito
Jun 8, 2012·Journal of the Royal Society, Interface·Antonio BoccaccioMarco De Spirito
Mar 29, 2020·Molecular Reproduction and Development·Janice P Evans
Oct 16, 2009·Journal of the Royal Society, Interface·Morteza KhalilianPoopak Eftekhari Yazdi
May 6, 2004·Journal of Cell Science·Tim WhalleyJoshua Zimmerberg
Oct 20, 2006·Theriogenology·David K GardnerMark G Larman
Apr 30, 2011·ACS Chemical Biology·Alison M KimThomas V O'Halloran

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