Dynamics of dissemination and outer surface protein expression of different European Borrelia burgdorferi sensu lato strains in artificially infected Ixodes ricinus nymphs.

Journal of Clinical Microbiology
V FingerleB Wilske

Abstract

Unfed Ixodes ricinus nymphs were infected with eight different strains and clones of Borrelia afzelii and B. garinii by capillary feeding. Except one B. afzelii clone, all expressed OspC in culture. Tick midguts and salivary glands were investigated at different time intervals for the presence of borreliae and for OspA and OspC phenotypes by immunofluorescence with simultaneous staining of OspA and OspC with monoclonal antibodies. Both species were transmittable to I. ricinus. All OspC-expressing strains and clones were able to disseminate into the salivary glands. In contrast, the OspC-negative B. afzelii clone was not detectable in the salivary glands, an indication that OspC plays an important role in dissemination. OspA-positive borreliae prevailed in the midgut. OspC positives were more frequent in the salivary glands than in the midgut. Notably, simultaneously OspA- and OspC-negative borreliae were detected in both organs. Kinetics of dissemination varied with the strains. The OspC-positive B. afzelii clone and all B. garinii OspA type 4 strains were detectable in the salivary glands right after feeding, while one B. garinii OspA type 6 strain invaded the salivary glands with a delay of 24 h. These findings support the hy...Continue Reading

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Citations

Feb 23, 2010·The Journal of Infectious Diseases·Manish KumarUtpal Pal
Sep 23, 2006·Vector Borne and Zoonotic Diseases·Juraj KociMilan Labuda
May 6, 2008·Vector Borne and Zoonotic Diseases·Bradley S SchneiderNordin S Zeidner
Nov 17, 2009·Nucleic Acids Research·Melisa S MedranoJenifer Coburn
Jan 15, 2004·The Journal of Clinical Investigation·Utpal PalErol Fikrig
Aug 2, 2011·Annual Review of Microbiology·D Scott Samuels
Feb 3, 2007·International Journal of Medical Microbiology : IJMM·Volker FingerleUlrike Schulte-Spechtel
Nov 29, 2015·Brazilian Journal of Otorhinolaryngology·Ozan GökdoğanZati Vatansever
Jun 7, 2003·Molecular Microbiology·Janet AlversonD Scott Samuels
Sep 1, 2004·The Lancet Infectious Diseases·Sunit Kumar Singh, Hermann Josef Girschick
Jun 9, 2005·Parasitology·J Piesman, L Gern
Feb 28, 2009·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Isis AbelAdivaldo H Fonseca

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