Dynamics of Fos-Jun-NFAT1 complexes

Proceedings of the National Academy of Sciences of the United States of America
V R Ramirez-Carrozzi, T K Kerppola

Abstract

Transcription initiation in eukaryotes is controlled by nucleoprotein complexes formed through cooperative interactions among multiple transcription regulatory proteins. These complexes may be assembled via stochastic collisions or defined pathways. We investigated the dynamics of Fos-Jun-NFAT1 complexes by using a multicolor fluorescence resonance energy transfer assay. Fos-Jun heterodimers can bind to AP-1 sites in two opposite orientations, only one of which is populated in mature Fos-Jun-NFAT1 complexes. We studied the reversal of Fos-Jun binding orientation in response to NFAT1 by measuring the efficiencies of energy transfer from donor fluorophores linked to opposite ends of an oligonucleotide to an acceptor fluorophore linked to one subunit of the heterodimer. The reorientation of Fos-Jun by NFAT1 was not inhibited by competitor oligonucleotides or heterodimers. The rate of Fos-Jun reorientation was faster than the rate of heterodimer dissociation at some binding sites. The facilitated reorientation of Fos-Jun heterodimers therefore can enhance the efficiency of Fos-Jun-NFAT1 complex formation. We also examined the influence of the preferred orientation of Fos-Jun binding on the stability and transcriptional activity of ...Continue Reading

References

Aug 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·L Stryer, R P Haugland
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·C LuoA Rao
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·D A LeonardT K Kerppola
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·R J DieboldT K Kerppola
Oct 23, 1998·Nature Structural Biology·D A Leonard, T K Kerppola
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·M ChytilG L Verdine
Jan 12, 2001·Journal of Molecular Biology·V R Ramirez-Carrozzi, T K Kerppola

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Citations

Jun 1, 2005·Biochemical and Biophysical Research Communications·Barbara CamuzeauxMartine Duterque-Coquillaud
Nov 6, 2007·Molecular Biology of the Cell·Grzegorz T GurdaJohn A Williams
Feb 18, 2003·Molecular and Cellular Biology·Vladimir Ramirez-Carrozzi, Tom Kerppola
Apr 5, 2008·Trends in Endocrinology and Metabolism : TEM·Ping LiuThorsten Wohland
Jan 8, 2014·Journal of Peptide Science : an Official Publication of the European Peptide Society·Stephanie VossYao-Wen Wu
Dec 9, 2014·Cell Cycle·Luca PuzziMauro Giacca
Aug 10, 2004·Biophysical Journal·Sungchul HohngTaekjip Ha
Aug 18, 2010·Biophysical Journal·Yuansheng SunAmmasi Periasamy
Jul 12, 2005·Experimental Cell Research·Anne-Mari HåkelienPhilippe Collas
Feb 24, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Yuansheng SunAmmasi Periasamy
Mar 22, 2001·The Journal of Biological Chemistry·V R Ramirez-Carrozzi, T K Kerppola
Jun 28, 2002·The Journal of Biological Chemistry·Kristen E BowerKathleen L McGuire
Mar 26, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Károly SzuhaiPancras C W Hogendoorn
Mar 15, 2008·Journal of the American Chemical Society·Huachang LuChristof M Niemeyer
May 24, 2019·The Journal of Physical Chemistry. B·Anders BarthDon C Lamb
Oct 13, 2007·Journal of the American Chemical Society·Kevin R KupchoKurt W Vogel

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