Dynamics of gapped DNA recognition by human polymerase beta

The Journal of Biological Chemistry
Maria J JezewskaWlodzimierz Bujalowski

Abstract

Kinetics of human polymerase beta binding to gapped DNA substrates having single stranded (ss) DNA gaps with five or two nucleotide residues in the ssDNA gap has been examined, using the fluorescence stopped-flow technique. The mechanism of the recognition does not depend on the length of the ssDNA gap. Formation of the enzyme complex with both DNA substrates occurs by a minimum three-step reaction, with the bimolecular step followed by two isomerization steps. The results indicate that the polymerase initiates the association with gapped DNA substrates through the DNA-binding subsite located on the 8-kDa domain of the enzyme. This first association step is independent of the length of the ssDNA gap and is characterized by similar rate constants for both examined DNA substrates. The subsequent, first-order transition occurs at the rate of approximately 600-1200 s(-1). This is the major docking step accompanied by favorable free energy changes in which the 31-kDa domain engages in interactions with the DNA. The 5'-terminal PO(4)(-) group downstream from the primer is not a specific recognition element of the gap. However, the phosphate group affects the enzyme orientation in the complex with the DNA, particularly, for the substr...Continue Reading

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Citations

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Aug 6, 2020·Genes·Anna V YudkinaDmitry O Zharkov
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Nov 10, 2004·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Leon P OehlersRonald B Walter
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Feb 28, 2004·Analytical Chemistry·Victor OkhoninSergey N Krylov

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