Dynamics of GATA1 binding and expression response in a GATA1-induced erythroid differentiation system

Genomics Data
Deepti JainRoss C Hardison

Abstract

During the maturation phase of mammalian erythroid differentiation, highly proliferative cells committed to the erythroid lineage undergo dramatic changes in morphology and function to produce circulating, enucleated erythrocytes. These changes are caused by equally dramatic alterations in gene expression, which in turn are driven by changes in the abundance and binding patterns of transcription factors such as GATA1. We have studied the dynamics of GATA1 binding by ChIP-seq and the global expression responses by RNA-seq in a GATA1-dependent mouse cell line model for erythroid maturation, in both cases examining seven progressive stages during differentiation. Analyses of these data should provide insights both into mechanisms of regulation (early versus late targets) and the consequences in cell physiology (e.g. distinctive categories of genes regulated at progressive stages of differentiation). The data are deposited in the Gene Expression Omnibus, series GSE36029, GSE40522, GSE49847, and GSE51338.

References

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Citations

Apr 15, 2008·Blood Cells, Molecules & Diseases·Clifford M TakemotoMichael A McDevitt
Dec 30, 2014·Molecular Aspects of Medicine·Alessandra di MasiClara Nervi
Jul 22, 2017·PloS One·Kevin R GillinderAndrew C Perkins
Oct 28, 2015·Molecular and Cellular Biology·G Celine HanB Franklin Pugh
Jun 28, 2019·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Manan ShahMerlin Crossley
May 26, 2017·Nucleic Acids Research·Melissa D IlsleyAndrew C Perkins

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Datasets Mentioned

BETA
GSE49847

Methods Mentioned

BETA
ChIP-seq
RNA-seq
immunoprecipitation
ChIP
PCR
Illumina sequencing
Assay
RNA

Software Mentioned

broadPeak
MACS
fastq
Bowtie
Snora
TopHat
Galaxy
UCSC Table Browser
FASTQ Groomer
ENCODE

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