Dynamics of glucose-induced localization of PKC isoenzymes in pancreatic beta-cells: diabetes-related changes in the GK rat

Diabetes
N WarwarR Nesher

Abstract

Glucose metabolism affects most major signal pathways in pancreatic beta-cells. Multiple protein kinases, including protein kinase C (PKC) isoenzymes, are involved in these effects; however, their role is poorly defined. Moreover, the dynamics of kinase isoenzyme activation in reference to the biphasic insulin secretion is unknown. In perfused pancreas of Wistar rats, PKCalpha staining was strongly associated with insulin staining, jointly accumulating in the vicinity of the plasma membrane during early first-phase insulin response. The signal declined before the onset of second phase and reappeared during second-phase insulin release as foci, only weekly associated with insulin staining; this signal persisted for at least 15 min after glucose stimulation. In the GK rat, glucose had minimal effect on beta-cell PKCalpha. In control beta-cells, PKCdelta stained as granulated foci with partial association with insulin staining; however, no glucose-dependent translocation was observed. In the GK rat, only minimal staining for PKCdelta was observed, increasing exclusively during early first-phase secretion. In Wistar beta-cells, PKCepsilon concentrated near the nucleus, strongly associated with insulin staining, with dynamics resemb...Continue Reading

References

Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S GanesanH Rasmussen
Jul 26, 1996·The Journal of Biological Chemistry·J T DeeneyP O Berggren
Jan 17, 1997·The Journal of Biological Chemistry·M YedovitzkyR Nesher
Feb 21, 1997·The Journal of Biological Chemistry·S VijayaraghavanD W Carr
Aug 25, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·E C DempseyR O Messing
May 1, 2001·Journal of Molecular Biology·A FelicielloE V Avvedimento
Apr 25, 2002·The Journal of Biological Chemistry·Melanie TardifJean Gosselin
Jun 28, 2002·Frontiers in Bioscience : a Journal and Virtual Library·D Page Baluch, David G Capco
Sep 6, 2002·Biochemical Pharmacology·Jacques PouysségurPhilippe Lenormand
Oct 12, 2002·American Journal of Physiology. Endocrinology and Metabolism·Gordon C YaneyBarbara E Corkey
Dec 12, 2002·The Journal of Endocrinology·P C GuestJ C Hutton
Feb 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Hewang LiMartha Vaughan
Apr 3, 2003·Immunological Reviews·Amnon Altman, Martin Villalba
Jun 5, 2003·The Journal of Experimental Medicine·Christa PfeifhoferGottfried Baier
Jul 16, 2003·Cellular and Molecular Life Sciences : CMLS·I GutcherN G Anderson
Jul 23, 2003·The Journal of Biological Chemistry·Ellen J TisdaleCristina R Artalejo
Aug 28, 2003·The Journal of Biological Chemistry·Carlos F MendezAlejandro M Bertorello
Feb 27, 2004·The Journal of Biological Chemistry·James G BurchfieldCarsten Schmitz-Peiffer
May 20, 2004·The Journal of Biological Chemistry·Kishore K SrivastavaLeonidas C Platanias

❮ Previous
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Citations

Dec 23, 2009·The Journal of Biological Chemistry·Paul T MannaAsipu Sivaprasadarao
May 31, 2007·The Journal of General Physiology·Tobias RoseMarjan Rupnik
Nov 6, 2008·Acta Physiologica·M Rupnik
Nov 28, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Miho OyasuNaoaki Saito
Dec 6, 2008·Trends in Endocrinology and Metabolism : TEM·Eric N ChurchillDaria Mochly-Rosen
Oct 9, 2007·Diabetes, Obesity & Metabolism·C-G Ostenson, S Efendic
Jan 8, 2009·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·T EdholmP M Hellström
Feb 5, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Elisa Fernández-MillánMaría Ángeles Martín
May 20, 2008·Biochimica Et Biophysica Acta·Nasim WarwarRafael Nesher
Apr 25, 2017·Biochimica Et Biophysica Acta. General Subjects·Yun XieGuang Ning
Dec 1, 2006·The Journal of Biological Chemistry·Toyoyoshi UchidaHirotaka Watada
Dec 9, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jaime Santo-DomingoAndreas Wiederkehr
Sep 17, 2008·Biochemical Society Transactions·Trevor J BidenLee Carpenter
Feb 13, 2021·Antioxidants·Petr JežekLydie Plecitá-Hlavatá

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