Dynamics of human DNA topoisomerases IIalpha and IIbeta in living cells

The Journal of Cell Biology
Morten O ChristensenChristian Mielke

Abstract

DNA topoisomerase (topo) II catalyses topological genomic changes essential for many DNA metabolic processes. It is also regarded as a structural component of the nuclear matrix in interphase and the mitotic chromosome scaffold. Mammals have two isoforms (alpha and beta) with similar properties in vitro. Here, we investigated their properties in living and proliferating cells, stably expressing biofluorescent chimera of the human isozymes. Topo IIalpha and IIbeta behaved similarly in interphase but differently in mitosis, where only topo IIalpha was chromosome associated to a major part. During interphase, both isozymes joined in nucleolar reassembly and accumulated in nucleoli, which seemed not to involve catalytic DNA turnover because treatment with teniposide (stabilizing covalent catalytic DNA intermediates of topo II) relocated the bulk of the enzymes from the nucleoli to nucleoplasmic granules. Photobleaching revealed that the entire complement of both isozymes was completely mobile and free to exchange between nuclear subcompartments in interphase. In chromosomes, topo IIalpha was also completely mobile and had a uniform distribution. However, hypotonic cell lysis triggered an axial pattern. These observations suggest th...Continue Reading

References

Jan 1, 1989·Annual Review of Biochemistry·L F Liu
May 1, 1985·The Journal of Cell Biology·W C EarnshawL F Liu
May 1, 1985·The Journal of Cell Biology·W C Earnshaw, M M Heck
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M BerriosP A Fisher
Apr 20, 1986·Journal of Molecular Biology·S M GasserU K Laemmli
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S TaageperaG J Gorbsky
Jan 1, 1996·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·A T Sumner
Aug 30, 1996·The Journal of Biological Chemistry·K KimuraA Kikuchi
Jan 1, 1996·Annual Review of Biochemistry·J C Wang
Sep 1, 1996·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·N ChalyD L Brown
Nov 1, 1996·Journal of Cellular Biochemistry·N Chaly, D L Brown
Feb 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P E Warburton, W C Earnshaw
Jun 19, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C A Austin, K L Marsh
Mar 24, 1999·Trends in Cell Biology·J White, E Stelzer
Dec 30, 1999·Science·X YangJ C Wang
Mar 11, 2000·Molecular Biology of the Cell·T Pederson
Apr 8, 2000·Trends in Cell Biology·M O OlsonA Szebeni
Apr 15, 2000·Nature·R D Phair, T Misteli
Jul 13, 2000·The Journal of Cell Biology·L S Shopland, J B Lawrence

❮ Previous
Next ❯

Citations

Feb 4, 2009·European Biophysics Journal : EBJ·Isaac GállegoJoan-Ramon Daban
Sep 20, 2005·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Jennifer M SpenceChristine J Farr
May 8, 2008·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·John F Marko
Dec 25, 2010·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Martin E van RoyenAdriaan B Houtsmuller
Apr 5, 2011·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Sandra C Moser, Jason R Swedlow
Mar 1, 2012·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Atocha RomeroMiguel Martín
Dec 11, 2002·Current Opinion in Cell Biology·Francisco J Iborra, Peter R Cook
Jun 6, 2003·Current Opinion in Cell Biology·Andrew Belmont
Apr 4, 2003·Current Opinion in Genetics & Development·Brian McHugh, Margarete M S Heck
Feb 26, 2008·Chemical Research in Toxicology·Omari J Bandele, Neil Osheroff
Apr 21, 2009·Nature Reviews. Cancer·John L Nitiss
Aug 13, 2010·Metallomics : Integrated Biometal Science·Joseph E Deweese, Neil Osheroff
Oct 2, 2003·European Journal of Biochemistry·Robert L LowDavid B Friedman
Dec 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Mikhail EltsovJacques Dubochet
Jul 4, 2008·The Journal of Biological Chemistry·Eriko ToyodaNoritaka Adachi
Jul 23, 2004·Omics : a Journal of Integrative Biology·Chaitanya A AthaleChristian Mielke
Feb 24, 2004·Molecular Biology of the Cell·Marta AgostinhoJoão Ferreira
Dec 6, 2002·Nucleic Acids Research·Ciaran MorrisonWilliam C Earnshaw
May 14, 2004·Nucleic Acids Research·Giulia MeariniFrank O Fackelmayer
Mar 22, 2006·Nucleic Acids Research·Kathryn L GilroyCaroline A Austin
Jun 15, 2007·Nucleic Acids Research·Jessica P WylesSusan P C Cole
Jul 20, 2007·Nucleic Acids Research·Michal StrosFrançois Strauss
Dec 2, 2008·Nucleic Acids Research·Joseph E Deweese, Neil Osheroff
Dec 9, 2008·Nucleic Acids Research·Joaquim Roca
Jun 6, 2009·Nucleic Acids Research·Mark JohnsonChristine J Farr
May 31, 2012·Molekuliarnaia biologiia·D A Giliarov, I S Shkundina
Dec 28, 2002·Cell Structure and Function·Jason R Swedlow, Melpomeni Platani
Aug 30, 2008·PLoS Biology·Paula A CoelhoClaudio E Sunkel
Jan 5, 2012·PloS One·Yang Zhang, Dieter W Heermann
Jan 31, 2014·Nucleic Acids Research·Christine J FarrAndrew C G Porter
Dec 4, 2002·Proceedings of the National Academy of Sciences of the United States of America·Andrew S Belmont
Mar 19, 2013·Annual Review of Biochemistry·Stefanie Hartman ChenTao-shih Hsieh
Mar 3, 2010·The Journal of Cell Biology·Ryo KawamuraJohn F Marko
Nov 21, 2012·The Journal of Cell Biology·Kumiko SamejimaWilliam C Earnshaw
Feb 16, 2016·Archives of Biochemistry and Biophysics·Anil ChekuriAnand K Kondapi
Nov 5, 2003·The Journal of Cell Biology·Yoshiaki AzumaMary Dasso

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Methods Mentioned

BETA
transfection
fluorescence studies
immunoprecipitation
confocal microscopy
PCR

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