Dynamics of human replication protein A subunit distribution and partitioning in the cell cycle

Experimental Cell Research
K G MurtiS H Lee

Abstract

Human replication protein A (RPA) is a three-sub-unit protein complex involved in DNA replication, repair, and recombination. To gain insight into the dynamics of subunit assembly, we examined the subcellular distribution of RPA subunits (p70, p34, and p11) during the cell cycle. All three subunits colocalized in G1 and S phases, showing a diffuse nuclear distribution in G1 but a dot-like nuclear pattern in S phase. During S phase, the subunits showed a pattern reminiscent of the replication granules/factories described by others as sites of replication machinery. In meta-phase, p70 preferentially associated with the spindle poles, p34 was found on chromosomes, and p11 remained in the cytoplasm. In telophase, p70 and p34 appeared in the forming daughter nuclei; p11 remained in the cytoplasm until G1. Among the three subunits only p34 was associated with the nuclear matrix and this association persisted throughout the cell cycle. We conclude that (i) RPA complex assembly is differentially regulated, (ii) the replication machinery may be anchored to the nuclear matrix, and (iii) RPA subunits partition during mitosis and sort into daughter nuclei by different routes.

Citations

Jan 12, 1999·The Biochemical Journal·J DongS H Lee
Feb 12, 2008·The Journal of Biological Chemistry·Brian D BeckSuk-Hee Lee
May 13, 2008·The Journal of Biological Chemistry·Stuart J HaringMarc S Wold
Dec 18, 2009·BMC Cell Biology·Zoltan CseresnyesCatherine M Green
Jul 6, 2014·Stem Cells and Development·Sang-Wook YoonKyung-Soon Park
Aug 25, 1999·The Journal of Cell Biology·D S DimitrovaD M Gilbert
Apr 16, 2003·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Noriko SatoHisao Masai
Jan 21, 2000·Experimental Cell Research·D S Dimitrova, D M Gilbert
Mar 15, 2015·Nucleic Acids Research·Jennifer M MasonDouglas K Bishop
Feb 9, 2002·Journal of Cellular Biochemistry·Shailesh K Choudhary, Rong Li
Apr 17, 2001·Radiation Research·S H MacPhail, P L Olive
Nov 18, 2003·The Journal of Biological Chemistry·Su-Jung ParkSuk-Hee Lee
Nov 21, 1998·The Journal of Biological Chemistry·K TreunerR Knippers
Jun 5, 2001·The Journal of Biological Chemistry·Y WangG Iliakis
Jul 27, 2000·Annals of the New York Academy of Sciences·J O NehlinV A Bohr
Jul 1, 1997·Molecular and Cellular Biology·C Iftode, J A Borowiec
Oct 1, 2004·Journal of Cell Science·Patricia FrançonMarcel Méchali

❮ Previous
Next ❯