PMID: 6107327Nov 1, 1980Paper

Dynamics of leukotriene C production by macrophages

The Journal of Experimental Medicine
C A RouzerZ A Cohn

Abstract

A method for the radiochemical assay of LTC production by mouse peritoneal macrophages in vitro is presented. The method involves labeling macrophages in culture with [5,6,8,9,11,12,14,15-3H]20:4 followed by stimulation of arachidonic acid (20:4) release under the experimental conditions desired. Radiolabeled leukotriene C (LTC) is recovered from the culture medium by extraction and silicic acid chromatography in 40% yield with full retention of biological activity. Because this LTC is radiochemically pure, the quantity of LTC release may be estimated from the amount of radioactivity in the sample. Use of the radioassay to study parameters affecting LTC synthesis by macrophages indicated that the time course of LTC synthesis and its relationship to the dose of a phagocytic stimulus (zymosan) were very similar to those of prostaglandin (PG) release. LTC release was also similar to that of PG in that lower levels of both metabolites were produced by Corynebacterium parvum-elicited macrophages than by resident cells. Finally, LTC release was stimulated in response to a challenge with antigen-antibody complexes, but lower maximal levels were attained than those with zymosan. The data presented here are consistent with the hypothesi...Continue Reading

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Citations

Jul 1, 1986·Digestive Diseases and Sciences·S E KirkhamW A Walker
Jan 1, 1984·Folia Microbiologica·L Fornůsek, V Vĕtvicka
Mar 31, 1987·European Journal of Pharmacology·E DavenasJ Benveniste
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May 1, 1986·Prostaglandins·J SraerR Ardaillou
Jan 1, 1985·Comparative Immunology, Microbiology and Infectious Diseases·R Roubin, J Benveniste
Jan 15, 2011·Analytical Chemistry·Heping YanXiangqun Zeng
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C A RouzerZ A Cohn
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C A RouzerZ A Cohn
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·E B CramerW A Scott
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J K Czop, K F Austen
Aug 1, 1981·The Journal of Experimental Medicine·G Boltz-NitulescuH L Spiegelberg
Feb 1, 1982·The Journal of Experimental Medicine·A JörgS J Klebanoff
Feb 1, 1982·The Journal of Experimental Medicine·W A ScottZ A Cohn
Mar 1, 1982·The Journal of Experimental Medicine·C A RouzerZ A Cohn
Apr 1, 1982·The Journal of Experimental Medicine·W A ScottZ A Cohn
Jun 1, 1982·The Journal of Experimental Medicine·N A PawlowskiZ A Cohn
Aug 1, 1983·The Journal of Experimental Medicine·N A PawlowskiW A Scott
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Sep 15, 1982·International Journal of Cancer. Journal International Du Cancer·D M ThomsonM K Bach

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