Dynamics of male canine germ cell development

PloS One
Aline F de SouzaDaniele S Martins

Abstract

Primordial germ cells (PGCs) are precursors of gametes that can generate new individuals throughout life in both males and females. Additionally, PGCs have been shown to differentiate into embryonic germ cells (EGCs) after in vitro culture. Most studies investigating germinative cells have been performed in rodents and humans but not dogs (Canis lupus familiaris). Here, we elucidated the dynamics of the expression of pluripotent (POU5F1 and NANOG), germline (DDX4, DAZL and DPPA3), and epigenetic (5mC, 5hmC, H3K27me3 and H3K9me2) markers that are important for the development of male canine germ cells during the early (22-30 days post-fertilization (dpf)), middle (35-40 dpf) and late (45-50 dpf) gestational periods. We performed sex genotype characterization, immunofluorescence, immunohistochemistry, and quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) analyses. Furthermore, in a preliminary study, we evaluated the capacity of canine embryo PGCs (30 dpf) to differentiate into EGCs. To confirm the canine EGCs phenotype, we performed alkaline phosphatase detection, immunohistochemistry, electron and transmission scanning microscopy and RT-qPCR analyses. The PGCs were positive for POU5F1 and H3K27me3 during al...Continue Reading

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Citations

Nov 13, 2018·Journal of Cellular Biochemistry·Shuang ZhaoNa Liu
Mar 25, 2019·Reproduction in Domestic Animals = Zuchthygiene·Ľubica HorňákováKatarína Šiviková
Apr 22, 2019·Reproduction in Domestic Animals = Zuchthygiene·Aline F de SouzaDaniele S Martins
Jan 9, 2020·Reproduction in Domestic Animals = Zuchthygiene·Stefano P MarelliMaria G Strillacci
Nov 30, 2019·Reproduction in Domestic Animals = Zuchthygiene·Angelika Chłopik, Anna Wysokińska
May 23, 2020·Stem Cells and Cloning : Advances and Applications·Alessandra de Oliveira PinheiroCarlos E Ambrosio
Oct 14, 2020·Journal of Tissue Engineering and Regenerative Medicine·Lucas Simões MachadoFabiana Fernandes Bressan
Mar 7, 2021·Animals : an Open Access Journal From MDPI·Aline Fernanda de SouzaDaniele Dos Santos Martins
Jun 12, 2021·Stem Cell Reviews and Reports·Naira Caroline Godoy PieriAndré Furugen Cesar de Andrade

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Methods Mentioned

BETA
transgenic
hysterectomies
genotyping
PCR
dissection
mechanical
enzymatic dissociation
mechanical dissociation
transmission
scanning electron microscopy

Software Mentioned

AxioVision
R
ImageJ
LinReg PCR
MEGA
BLAST

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