Dynamics of membrane adhesion: the role of polyethylene glycol spacers, ligand-receptor bond strength, and rupture pathway

Langmuir : the ACS Journal of Surfaces and Colloids
Joyce Y Wong, Tonya L Kuhl

Abstract

Biological adhesion typically occurs through discrete cross bridges between complementary molecules on adjacent membranes. Here we report quantitative measurements of the binding distance between a lipid membrane functionalized with ligands on flexible polymer tether chains and a second membrane bearing complementary receptors using the surface force apparatus technique. The binding distance is shown to increase as a function of polymer tether length. Upon separation, adhesive failure occurs not at the strong ligand-receptor bond but primarily through the mechanical pullout of cross-bridging polymer tethers from the membrane. We summarize these measurements of complementary membrane adhesion dynamics using an energy-state diagram that encompasses the energetics of the polymer tether, ligand-receptor bond strength, and number of cross bridges formed.

References

Aug 14, 1992·Biochimica Et Biophysica Acta·M C Woodle, D D Lasic
Aug 2, 1990·Nature·T A Springer
Apr 14, 1995·Science·E A Clark, J S Brugge
Apr 15, 1994·Science·E L FlorinH E Gaub
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·S R Sheth, D Leckband
Jun 6, 2000·Advanced Drug Delivery Reviews·M C Woodle
Jul 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·J SatulovskyI Szleifer
May 8, 2001·Annual Review of Biophysics and Biomolecular Structure·E Evans
Jan 5, 2002·Quarterly Reviews of Biophysics·D Leckband, J Israelachvili
Dec 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Harshad S SakhalkarDouglas J Goetz
Mar 26, 2004·Biophysical Journal·Timothy V RattoMichael W McElfresh
Jul 23, 2004·The Journal of Chemical Physics·Andre G Moreira, Carlos M Marques
Feb 3, 2005·Nature Reviews. Drug Discovery·Vladimir P Torchilin
May 12, 1978·Science·L J Carter

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Citations

Oct 7, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Matthew C HagyElena E Dormidontova
Jan 1, 2010·Annual Review of Chemical and Biomolecular Engineering·Deborah Leckband
Aug 8, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Peter J Onyskiw, Omolola Eniola-Adefeso
Sep 28, 2010·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Thomas SpeckUdo Seifert
Aug 14, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Gabriel S LongoI Szleifer

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