Dynamics of microvascular oxygen pressure across the rest-exercise transition in rat skeletal muscle

Respiration Physiology
Brad J BehnkeDavid C Poole

Abstract

There exists substantial controversy as to whether muscle oxygen (O2) delivery (QO2) or muscle mitochondrial O2 demand determines the profile of pulmonary VO2 kinetics in the rest-exercise transition. To address this issue, we adapted intravascular phosphorescence quenching techniques for measurement of rat spinotrapezius microvascular O2 pressure (PO2m). The spinotrapezius muscle intravital microscopy preparation is used extensively for investigation of muscle microcirculatory control. The phosphor palladium-meso-tetra(4-carboxyphenyl)porphyrin dendrimer (R2) at 15 mg/kg was bound to albumin within the blood of female Sprague-Dawley rats ( approximately 250 g). Spinotrapezius blood flow (radioactive microspheres) and PO2m profiles were determined in situ across the transition from rest to 1 Hz twitch contractions. Stimulation increased muscle blood flow by 240% from 16.6 +/- 3.0 to 56.2 +/- 8.3 (SE) ml/min per 100 g (P < 0.05). Muscle contractions reduced PO2m from a baseline of 31.4 +/- 1.6 to a steady-state value of 21.0 +/- 1.7 mmHg (n = 24, P < 0.01). The response profile of PO2m was well fit by a time delay of 19.2+/-2.8 sec (P < 0.05) followed by a monoexponential decline (time constant, 21.7 +/- 2.1 sec) to its steady s...Continue Reading

References

Dec 1, 1979·Clinical Obstetrics and Gynecology
Dec 1, 1995·The Journal of Clinical Investigation·H SuzukiG W Schmid-Schönbein
Apr 12, 2000·Acta Physiologica Scandinavica·J Bangsbo

❮ Previous
Next ❯

Citations

Apr 5, 2011·European Journal of Applied Physiology·Juan M MuriasDonald H Paterson
Sep 20, 2011·European Journal of Applied Physiology·Glen R BelfryScott G Thomas
Nov 12, 2002·Respiratory Physiology & Neurobiology·Brad J BehnkeDavid C Poole
Aug 1, 2012·Critical Care Medicine·Robert T DavisBradley J Behnke
Feb 2, 2011·Acta Physiologica·R N Pittman
Nov 14, 2002·Canadian Journal of Applied Physiology = Revue Canadienne De Physiologie Appliquée·Tara L Haas
Dec 7, 2007·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Darren S DeLoreyJohn M Kowalchuk
Aug 28, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Daniel M HiraiDavid C Poole
Nov 13, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Steven W CoppTimothy I Musch
Nov 22, 2011·American Journal of Physiology. Heart and Circulatory Physiology·David C PooleTimothy I Musch
Jun 17, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·James M DominguezBradley J Behnke
Jun 9, 2012·Journal of Applied Physiology·Daniel M HiraiDavid C Poole
Oct 3, 2009·Journal of Applied Physiology·Bradley J Behnke, Michael D Delp
Jan 8, 2011·Journal of Applied Physiology·Danielle J McCulloughBradley J Behnke
Aug 3, 2010·The Journal of Experimental Biology·Gerolf GrosThomas Jue
Sep 8, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Steven W CoppTimothy I Musch
Dec 20, 2015·Microvascular Research·William H NugentAleksander S Golub
Jan 8, 2005·The Journal of Physiology·Paul McDonoughDavid C Poole
Apr 15, 2003·The Journal of Physiology·Brad J BehnkeDavid C Poole
Jan 30, 2013·Respiratory Physiology & Neurobiology·Michelle L Davis, Thomas J Barstow
Jul 6, 2010·Respiratory Physiology & Neurobiology·Priscila B BarbosaLeonardo F Ferreira
Nov 26, 2015·Respiratory Physiology & Neurobiology·Clark T HoldsworthTimothy I Musch
Jul 22, 2009·Respiratory Physiology & Neurobiology·Brad J BehnkeDavid C Poole
Mar 20, 2012·Nitric Oxide : Biology and Chemistry·Matthias TotzeckTienush Rassaf
Dec 23, 2006·Experimental Physiology·David C Poole, Leonardo F Ferreira
Feb 15, 2005·Experimental Physiology·Leonardo F FerreiraThomas J Barstow
Apr 4, 2006·The Journal of Physiology·Leonardo F FerreiraDavid C Poole
Oct 1, 2011·Biotechnology and Bioengineering·S K DasikaB R Locke
Oct 3, 2012·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Roland N Pittman
Sep 3, 2013·Experimental Physiology·David C PooleTimothy I Musch
Mar 16, 2005·Respiratory Physiology & Neurobiology·Brad J BehnkeDavid C Poole
May 4, 2011·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Steven W CoppDavid C Poole

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.