PMID: 41360Jan 1, 1979

Dynamics of multiplication of BHK21C13 cells in a suspension

Veterinarno-meditsinski nauki
G TivchevE Veleva

Abstract

In order to master a new method for the production of a F. M. D. vaccine the authors have studied the reproduction dynamics of BHK21C13 cells in the conditions of a suspension culture. The culturing of the cells has been carried out under laboratory conditions, investigating also the concurrent changes in the pH value along with the cell dynamics. The nutrient medium used and the conditions of cultivation has made it possible to study the properties and obtain a qualitative cell suspension. The cells obtained have shown equally good growth in a monolayer medium and in a suspension, and are appropriate for the reproduction of the F. M. D. virus.

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