Dynamics of Salmonella infection of macrophages at the single cell level.

Journal of the Royal Society, Interface
Julia R GogClare E Bryant

Abstract

Salmonella enterica causes a range of diseases. Salmonellae are intracellular parasites of macrophages, and the control of bacteria within these cells is critical to surviving an infection. The dynamics of the bacteria invading, surviving, proliferating in and killing macrophages are central to disease pathogenesis. Fundamentally important parameters, however, such as the cellular infection rate, have not previously been calculated. We used two independent approaches to calculate the macrophage infection rate: mathematical modelling of Salmonella infection experiments, and analysis of real-time video microscopy of infection events. Cells repeatedly encounter salmonellae, with the bacteria often remain associated with the macrophage for more than ten seconds. Once Salmonella encounters a macrophage, the probability of that bacterium infecting the cell is remarkably low: less than 5%. The macrophage population is heterogeneous in terms of its susceptibility to the first infection event. Once infected, a macrophage can undergo further infection events, but these reinfection events occur at a lower rate than that of the primary infection.

References

Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·K Y Leung, B B Finlay
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P I FieldsF Heffron
Dec 23, 1999·The Journal of Biological Chemistry·L C AlldridgeC E Bryant
Nov 16, 2001·Seminars in Immunology·P Sansonetti
Jan 5, 2002·Microbes and Infection·J P Gorvel, S Méresse
May 22, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Matthew C J RoyleClare E Bryant
Oct 8, 2004·Cellular Microbiology·Karsten Hueffer, Jorge E Galán
Apr 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sabine TötemeyerClare Bryant
Aug 24, 2007·Cellular Microbiology·Susan L Fink, Brad T Cookson
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Fernando Oneissi MartinezMassimo Locati
Apr 23, 2008·Infection and Immunity·Jennifer L BishopB Brett Finlay
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sophie HelaineDavid W Holden

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Citations

Dec 14, 2012·Journal of the Royal Society, Interface·Olivier RestifPietro Mastroeni
Sep 27, 2013·PLoS Pathogens·Patrick KaiserRoland R Regoes
Nov 26, 2014·Proceedings of the National Academy of Sciences of the United States of America·Si Ming ManClare E Bryant
Jul 1, 2015·Bulletin of Mathematical Biology·Glenn YoungJonathan E Rubin
Sep 10, 2013·Biophysical Journal·Debjani PaulPietro Cicuta
Dec 24, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Sarra AchouriClare E Bryant
Aug 4, 2016·Autophagy·Noelia López-MonteroFrancisco García-Del Portillo
Nov 3, 2016·Gut Microbes·Roi Avraham, Deborah T Hung
Aug 31, 2016·Virulence·Estel Ramos-MarquèsFrancisco García-Del Portillo
Jun 13, 2017·The Journal of Infection·Noha A HassunaLynda J Partridge
Jun 24, 2015·Infection and Immunity·Kate M O'KeeffeRachel M McLoughlin
Aug 15, 2019·PLoS Neglected Tropical Diseases·Rosangela Salerno-GoncalvesMarcelo B Sztein
Sep 12, 2019·Cellular Microbiology·Erik Petersen, Samuel I Miller
Aug 2, 2019·Reports on Progress in Physics·Bortolo Matteo MognettiLorenzo Di Michele
Oct 11, 2019·Royal Society Open Science·Adedoyin Awofisayo-OkuyeluIan Hall
Aug 22, 2017·Reports on Progress in Physics·David M Richards, Robert G Endres
Jun 2, 2020·PLoS Computational Biology·Jonathan CarruthersCarmen Molina-París
Aug 27, 2017·Biochemical Society Transactions·Charlotte Macleod, Clare E Bryant
Dec 21, 2017·MBio·Sónia CastanheiraFrancisco García-Del Portillo
Apr 2, 2021·Nature Communications·Stefan OtteFernando Peruani
Jul 22, 2017·Microbiology Spectrum·M Graciela Pucciarelli, Francisco García-Del Portillo
Sep 4, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Snehal K ShuklaVivek Gupta

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

athematica
AF6000

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