Dynamics of single human embryonic stem cells and their pairs: a quantitative analysis

Scientific Reports
L E WadkinA Shukurov

Abstract

Numerous biological approaches are available to characterise the mechanisms which govern the formation of human embryonic stem cell (hESC) colonies. To understand how the kinematics of single and pairs of hESCs impact colony formation, we study their mobility characteristics using time-lapse imaging. We perform a detailed statistical analysis of their speed, survival, directionality, distance travelled and diffusivity. We confirm that single and pairs of cells migrate as a diffusive random walk for at least 7 hours of evolution. We show that the presence of Cell Tracer significantly reduces hESC mobility. Our results open the path to employ the theoretical framework of the diffusive random walk for the prognostic modelling and optimisation of the growth of hESC colonies. Indeed, we employ this random walk model to estimate the seeding density required to minimise the occurrence of hESC colonies arising from more than one founder cell and the minimal cell number needed for successful colony formation. Our prognostic model can be extended to investigate the kinematic behaviour of somatic cells emerging from hESC differentiation and to enable its wide application in phenotyping of pluripotent stem cells for large scale stem cell c...Continue Reading

References

Oct 12, 2000·Biophysical Journal·J P RieuY Sawada
Sep 8, 2004·Bio Systems·D C WalkerR H Smallwood
May 10, 2007·International Journal of Andrology·Neil J HarrisonPeter W Andrews
May 29, 2007·Nature Biotechnology·Kiichi WatanabeYoshiki Sasai
Sep 19, 2015·Scientific Reports·Smruti M PhadnisRenee A Reijo Pera

❮ Previous
Next ❯

Citations

Jun 14, 2018·Physical Biology·L E WadkinN G Parker
Jun 22, 2019·Biochemistry. Biokhimii︠a︡·E I UstyantsevaS M Zakian
Nov 28, 2019·Scientific Reports·Sirio Orozco-FuentesNicholas G Parker
Aug 18, 2020·SN Applied Sciences·L E WadkinN G Parker
Oct 28, 2019·Scientific Reports·L E WadkinA Shukurov

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.