DOI: 10.1101/505925Dec 26, 2018Paper

Dynamics of Spaetzle morphogen shuttling in the Drosophila embryo shapes pattern

BioRxiv : the Preprint Server for Biology
Neta RahimiBen-Zion Shilo

Abstract

Establishment of morphogen gradients in the early Drosophila embryo is challenged by a diffusible extracellular milieu, and rapid nuclear divisions that occur at the same time. To understand how a sharp gradient is formed within this dynamic environment, we followed the generation of graded nuclear Dorsal (Dl) protein, the hallmark of pattern formation along the dorso-ventral axis, in live embryos. We show that a sharp gradient is formed through extracellular, diffusion-based morphogen shuttling that progresses through several nuclear divisions. Perturbed shuttling in wntD mutant embryos results in a flat activation peak and aberrant gastrulation. Re-entry of Dl into the nuclei at each cycle refines the signaling output, by guiding graded accumulation of the T48 transcript that drives patterned gastrulation. We conclude that diffusion-based ligand shuttling, coupled with dynamic readout, establishes a refined pattern within the diffusible environment of early embryos.

Related Concepts

Cell Nucleus
Drosophila
Embryo
Environment
Ligands
Dl (dorsal) protein, Drosophila
Gastrulation
Shapes
Patterns
Extracellular

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