Dynamics of Sun5 localization during spermatogenesis in wild type and Dpy19l2 knock-out mice indicates that Sun5 is not involved in acrosome attachment to the nuclear envelope

PloS One
Sandra YassineChristophe Arnoult

Abstract

The acrosome is an organelle that is central to sperm physiology and a defective acrosome biogenesis leads to globozoospermia, a severe male infertility. The identification of the actors involved in acrosome biogenesis is therefore particularly important to decipher the molecular pathogeny of globozoospermia. We recently showed that a defect in the DPY19L2 gene is present in more than 70% of globozoospermic men and demonstrated that Dpy19l2, located in the inner nuclear membrane, is the first protein involved in the attachment of the acrosome to the nuclear envelope (NE). SUN proteins serve to link the nuclear envelope to the cytoskeleton and are therefore good candidates to participate in acrosome-nucleus attachment, potentially by interacting with DPY19L2. In order to characterize new actors of acrosomal attachment, we focused on Sun5 (also called Spag4l), which is highly expressed in male germ cells, and investigated its localization during spermatogenesis. Using immunohistochemistry and Western blot experiments in mice, we showed that Sun5 transits through different cellular compartments during meiosis. In pachytene spermatocytes, it is located in a membranous compartment different to the reticulum. In round spermatids, it ...Continue Reading

References

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Citations

Mar 29, 2016·Current Opinion in Cell Biology·David M Graham, Keith Burridge
May 26, 2017·Human Molecular Genetics·Razan A ElkhatibMichael J Mitchell
May 5, 2016·Biology Open·Pierre F RayChristophe Arnoult
Sep 26, 2017·ELife·Yongliang ShangWei Li
Jul 8, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Razan A ElkhatibCatherine Metzler-Guillemain
Feb 1, 2019·Biological Reviews of the Cambridge Philosophical Society·Cátia D PereiraSandra Rebelo
May 31, 2019·Acta Biochimica Et Biophysica Sinica·Xiaohua LiXiaowei Xing
May 31, 2017·EMBO Molecular Medicine·Zine-Eddine KherrafChristophe Arnoult
Mar 12, 2020·The Journal of Biological Chemistry·Qian GaoQinghua Shi
Apr 19, 2015·Human Reproduction Update·Charles CouttonPierre F Ray
Jun 28, 2018·Basic and Clinical Andrology·Marine PaciCatherine Metzler-Guillemain
Dec 3, 2020·International Journal of Molecular Sciences·Vera KmonickovaKaterina Komrskova
Nov 14, 2020·Best Practice & Research. Clinical Endocrinology & Metabolism·Julie BeuroisCharles Coutton
May 1, 2021·Genes·Francesco ManfrevolaRosanna Chianese
Jul 17, 2021·Frontiers in Cell and Developmental Biology·Yunfei ZhangXiaowei Xing
Aug 15, 2021·Nature Communications·Ying ZhangLi Yuan

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Datasets Mentioned

BETA
GM130

Methods Mentioned

BETA
confocal microscopy
glycosylation
deglycosylation
gene knock-out

Software Mentioned

Zen

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