PMID: 11906904Mar 22, 2002Paper

Dynamics of testicular germ cell proliferation in normal mice and transgenic mice overexpressing rat androgen-binding protein: a flow cytometric evaluation

Biology of Reproduction
D Antony JeyarajPeter Petrusz

Abstract

Transgenic mice carrying rat androgen-binding protein (ABP) genomic DNA express high amounts of testicular ABP and develop a progressive impairment of spermatogenesis. To understand the mechanism of these changes, we have studied the pattern of testicular germ cell proliferation from 7 to 360 days of age in wild-type (WT) control and transgenic homozygous (ABP-TG) mice by flow cytometry after labeling DNA in isolated germ cells with propidium iodide. At all ages studied, the body weight of the ABP-TG mice was lower than that of age-matched WT controls. Significantly reduced testicular weight and total germ cell number in the ABP-TG mice were evident from Day 30 and Day 60, respectively. Flow cytometric analysis of isolated germ cells revealed that the number of germ cells undergoing proliferation (S-phase cells) was identical in WT control and ABP-TG mice up to Day 14. Subsequently, the number of germ cells in S-phase was consistently higher in ABP-TG than in WT mice. The number of primary spermatocytes was significantly increased starting from Day 60, and the numbers of round and elongated spermatids were significantly reduced in the ABP-TG animals from Day 21 and Day 60 onwards, respectively. Immunocytometry for intracellular...Continue Reading

References

Jan 1, 1991·Annals of the New York Academy of Sciences·A GerardH Gerard
Dec 1, 1990·Endocrinology·B J DanzoH L Anthony
Sep 1, 1987·Journal of Reproduction and Fertility·M K HollandB J Danzo
Nov 14, 1973·Nature: New Biology·V HanssonF S French
Dec 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P OrdronneauP Petrusz
Dec 1, 1981·Journal of Steroid Biochemistry·G L GunsalusC W Bardin
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·A M NakhlaW Rosner
Jun 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·A Gerard
Jun 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·S LarribaJ Reventós
Nov 1, 1994·Biology of Reproduction·R I McLachlanD M Robertson

❮ Previous
Next ❯

Citations

Jul 2, 2003·Reproductive Biology and Endocrinology : RB&E·D Antony JeyarajPeter Petrusz
Jul 3, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Cleida A OliveiraRex A Hess
Nov 11, 2006·Proceedings of the National Academy of Sciences of the United States of America·Caixia ZhangChawnshang Chang
Feb 10, 2006·Annals of the New York Academy of Sciences·Jonathan P Jarow, Barry R Zirkin
Apr 12, 2011·Molecular Reproduction and Development·Fang LiuHui Huang
May 19, 2006·Molecular and Cellular Endocrinology·Mukhtar AleemManjeet K Gill-Sharma
Mar 17, 2009·Fertility and Sterility·Fabrizio AntonangeliClaudia Giampietri
Feb 20, 2018·Basic and Clinical Andrology·Katharina Gegenschatz-SchmidFaruk Hadziselimovic
Jun 21, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S V Tokalov, H O Gutzeit
Aug 30, 2008·Endocrinology·Sonja E GustinAndrew H Sinclair
Feb 21, 2018·Biology of Reproduction·Michael D Griswold

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis